CD9 Antibody (209306) [Unconjugated]

Human peripheral blood platelets were stained with Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB1880, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins.

Validation of protein expression by Western blotting.All four exosome samples and their corresponding cell lines were used for validation. Furthermore, supernatant from the pelleted exosomes was used as a control. The selected proteins FASN, XPO1, CD9 and PDCD6IP, were tested with ENO1 and GAPDH as controls. PSA was tested to confirm it is secreted through alternative secretion pathway and therefore not present within exosomes. The nearest protein marker (kDa) is indicated for each blot. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24391718), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of EGFP & associated EV markers M1-CD9truc-EGFP & their conditioned medium. (A) Illustration of our EV reporter gene & protein. The CD9truc-EGFP coding sequence is driven by the CMV promoter & encodes a fusion protein consisting of the first 3 transmembrane domains of CD9 & EGFP, enabling genetic labeling & EV surface display of EGFP. (B) M1 stable transfected with CD9truc-EGFP are green fluorescent in contrast to non-transfected M1 . Nuclei (blue) & GFP (green). n = 3, ×200 magnification (C) Immunofluorescence labeling of CD9 in M1-CD9truc-EGFP using Anti-hCD9 AB shows colocalization between CD9 (red), EGFP (green) & nuclei (blue). ×200 magnification (n = 3) (D) M1 stable transfected with CD9truc-EGFP expressing a band reacting with an anti-GFP antibody in & PEG-precipitated conditioned medium. Actin & Lamin A/C were detected in cell lysates only, & EVs markers ALIX & Flotillin were detected in conditioned medium from transfected & non-transfected M1 (n = 3). Full-length blots are shown in Supplementary Figure S2A-F. (E) Tunable resistive pulse sensing on conditioned medium from non-transfected M1 & stable transfected CD9truc-EGFP indicated that the size distribution of EVs is not affected the expression reporter proteins (n = 3). (F) Size-exclusion chromatography fractions of CD9truc-EGFP cell-conditioned medium showed full-length CD9truc-EGFP in fractions 1–3, while shedded EGFP was present in fractions 10–12 (n = 3). Full-length blots are shown in Supplementary Figure S2G. Below the WB, the protein concentration in each fraction is shown (G) Anti-GFP immunoprecipitation of conditioned medium co-isolates CD9truc-EGFP & EV markers ALIX & TSG101 only in M1 stable transfected with CD9truc-EGFP (n = 3). Full-length blots are shown in Supplementary Figure S3A-C. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35017633), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 209306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD9 Antibody (209306) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CD9
  Met1-Val228
  Accession # P21926
• Specificity: Detects human CD9 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD9 Antibody (209306) [Unconjugated]

• BA2
• BA-2/p24 antigen
• BTCC-1
• CD9 antigen
• CD9 molecule
• CD9
• Cell growth-inhibiting gene 2 protein
• DRAP-27
• Leukocyte antigen MIC3
• MIC3
• motility related protein-1,5H9 antigen
• Motility-related protein
• MRP-1FLJ99568
• p24
• tetraspanin-29
• TSPAN29
• TSPAN-29
• TSPAN29MIC3CD9 antigen (p24)

Background

CD9, also known as motility-related protein-1 (MRP-1), is a tetraspan family glycoprotein expressed by a variety of hematopoietic and epithelial cells. It forms homotypic associations as well as heterotypic associations with other tetraspan proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD9 Antibody (MAB1880)(16)

Publications using MAB1880

• Yoon, H;Park, S;Kwon, YR;Kim, YJ; Potential role of miR-29b from mesenchymal stromal cell-derived extracellular vesicles in leukemic cell progression PloS one 2025-09-10 [PMID: 40929300] (Western Blot, Human)
• Gratpain, V;Loriot, A;Bottemanne, P;d'Auria, L;Terrasi, R;Payen, VL;van Pesch, V;Muccioli, GG;des Rieux, A; Influence of a pro-inflammatory stimulus on the miRNA and lipid content of human dental stem cell-derived extracellular vesicles and their impact on microglial activation Heliyon 2024-03-15 [PMID: 38463764] (ELISA Capture, Human)
• Luong T. H. Nguyen, Jingjing Zhang, Xilal Y. Rima, Xinyu Wang, Kwang Joo Kwak, Tamio Okimoto et al. An immunogold single extracellular vesicular RNA and protein (Au SERP) biochip to predict responses to immunotherapy in non-small cell lung cancer patients Journal of Extracellular Vesicles 9/12/2022 [PMID: 36093740]
• Céline Vrancx, Devkee M. Vadukul, Nuria Suelves, Sabrina Contino, Ludovic D’Auria, Florian Perrin et al. Mechanism of Cellular Formation and In Vivo Seeding Effects of Hexameric beta -Amyloid Assemblies Molecular Neurobiology 12/1/2021 [PMID: 34608607]
• Alex P. Shephard, Peter Giles, Mariama Mbengue, Amr Alraies, Lisa K. Spary, Howard Kynaston et al. Stroma‐derived extracellular vesicle mRNA signatures inform histological nature of prostate cancer Journal of Extracellular Vesicles 10/1/2021 [PMID: 34596356]
• CM Bliss, AJ Parsons, R Nachbagaue, JR Hamilton, F Cappuccini, M Ulaszewska, JP Webber, A Clayton, AVS Hill, L Coughlan Targeting Antigen to the Surface of EVs Improves the In�Vivo Immunogenicity of Human and Non-human Adenoviral Vaccines in Mice Mol Ther Methods Clin Dev, 2019-12-24;16(0):108-125. 2019-12-24 [PMID: 31934599] (ELISA Capture, Human)
• V Bokun, JJ Moore, R Moore, CC Smallcombe, TJ Harford, F Rezaee, F Esper, G Piedimonte Respiratory syncytial virus exhibits differential tropism for distinct human placental cell types with Hofbauer cells acting as a permissive reservoir for infection PLoS ONE, 2019-12-02;14(12):e0225767. 2019-12-02 [PMID: 31790466] (Cell Purification, Human)
• Endika Prieto-Fernández, Ana María Aransay, Félix Royo, Esperanza González, Juan José Lozano, Borja Santos-Zorrozua et al. A Comprehensive Study of Vesicular and Non-Vesicular miRNAs from a Volume of Cerebrospinal Fluid Compatible with Clinical Practice Theranostics 6/19/2019 [PMID: 31367240]
• AC Brook, RH Jenkins, A Clayton, A Kift-Morga, AC Raby, AP Shephard, B Mariotti, SM Cuff, F Bazzoni, T Bowen, DJ Fraser, M Eberl Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis Sci Rep, 2019-07-12;9(1):10136. 2019-07-12 [PMID: 31300703] (ELISA Detection, Human)
• Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey Transfer of Functional Cargo in Exomeres Cell Rep, 2019-04-04;0(0):. 2019-04-04 [PMID: 30956133] (Western Blot, Human)
• Tanja Jankovic-Karasoulos, Dale McAninch, Dylan McCullough, Rebecca L. Wilson, Tina Bianco-Miotto, Claire T. Roberts Isolation of villous cytotrophoblasts from second trimester human placentas Placenta 12/1/2018 [PMID: 30541665]
• O Jaka, L Casas-Frai, M Azpitarte, A Aiastui, A López de M, A Sáenz FRZB and melusin, overexpressed in LGMD2A, regulate integrin ?1D isoform replacement altering myoblast fusion and the integrin-signalling pathway Expert Rev Mol Med, 2017-03-16;19(0):e2. 2017-03-16 [PMID: 28300015] (Western Blot, Human)
• V Domenyuk, Z Zhong, A Stark, N Xiao, HA O'Neill, X Wei, J Wang, TT Tinder, S Tonapi, J Duncan, T Hornung, A Hunter, MR Miglarese, J Schorr, DD Halbert, J Quackenbus, G Poste, DA Berry, G Mayer, M Famulok, D Spetzler Plasma Exosome Profiling of Cancer Patients by a Next Generation Systems Biology Approach Sci Rep, 2017-02-20;7(0):42741. 2017-02-20 [PMID: 28218293] (Western Blot, Human)
• Duijvesz D, Burnum-Johnson K, Gritsenko M, Hoogland A, Vredenbregt-van den Berg M, Willemsen R, Luider T, Pasa-Tolic L, Jenster G Proteomic profiling of exosomes leads to the identification of novel biomarkers for prostate cancer. PLoS ONE, 2013-12-31;8(12):e82589. 2013-12-31 [PMID: 24391718] (Western Blot, Human)
• David T. Madden, Diana Davila-Kruger, Simon Melov, Dale E. Bredesen Human Embryonic Stem Cells Express Elevated Levels of Multiple Pro-Apoptotic BCL-2 Family Members PLoS ONE 12/9/2011 [PMID: 22174832]
• Toti P, Arcuri F, Tang Z, Schatz F, Zambrano E, Mor G, Niven-Fairchild T, Abrahams VM, Krikun G, Lockwood CJ, Guller S Focal increases of fetal macrophages in placentas from pregnancies with histological chorioamnionitis: potential role of fibroblast monocyte chemotactic protein-1. Am. J. Reprod. Immunol., 2010-11-19;65(5):470-9. 2010-11-19 [PMID: 21087336] (Cell Selection, Human)

Bioinformatics

• Gene Symbol: CD9
• Uniprot:
  • P21926()