CD82/Kai-1 Antibody - BSA Free

Western Blot: CD82/Kai-1 Antibody [NBP1-76775] - A549 cell lysate with CD82 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry: CD82/Kai-1 Antibody - BSA Free [NBP1-76775] - Immunohistochemistry of CD82/Kai-1 in human colon tissue with CD82/Kai-1 antibody at 2.5 u/mL.

Immunocytochemistry/ Immunofluorescence: CD82/Kai-1 Antibody - BSA Free [NBP1-76775] - Immunofluorescence of CD82/Kai-1 in Human Colon cells with CD82/Kai-1 antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD82/Kai-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CD82/Kai-1 Antibody - BSA Free (NBP1-76775) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-CD82/Kai-1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human KAI1. The immunogen is located within the last 50 amino acids of KAI1. Amino Acid Squence: CRHVHSEDYSKVPKY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD82
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunoprecipitation
  • Western Blot 0.5-1 ug/ml
• Application Notes: Use in IP reported in scientific publication (PMID: 32573489).
• Control Peptide:

  CD82/Kai-1 Peptide (NBP1-76775PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CD82/Kai-1 Antibody - BSA Free

• CD82 antigenC33
• CD82 molecule
• CD82
• IA4
• IA4C33 antigen
• Inducible membrane protein R2
• KAI1
• KAI1GR15
• kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4))
• Metastasis suppressor Kangai-1
• R2
• SAR2
• SAR2tetraspanin-27
• ST6
• ST6tspan-27
• Suppressor of tumorigenicity 6 protein
• Tetraspanin-27
• TSPAN27
• Tspan-27
• TSPAN274F9

Background

KAI1 was initially identified from a T-cell activation study as a four-transmembrane protein that plays an accessory role in T-cell activation, and was later determined to act as a cancer metastasis suppressor gene. This protein is ubiquitously expressed at moderate to high levels in most tissues, but its expression is downregulated during tumor progression. The loss of KAI1 and p53 is associated with poor survival for prostate and other cancer patients. Recently, KAI1 was found to interact with DARC, the Duffy antigen for chemokines using a yeast two hybrid screen. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least two isoforms of KAI1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD82/Kai-1 Antibody (NBP1-76775)(2)

Publications using NBP1-76775

• Schweitzer KS, Jinawath N, Yonescu R et al. IGSF3 mutation identified in patient with severe COPD alters cell function and motility JCI Insight 2020-06-23 [PMID: 32573489] (WB, IP, Mouse)
• Xu XD, Shen HB, Zhu L et al. Anti-RhoC siRNAs inhibit the proliferation and invasiveness of breast cancer cells via modulating the KAI1, MMP9, and CXCR4 expression. Onco Targets Ther. 2017-04-03 [PMID: 28367066] (WB, Human)

Bioinformatics

• Gene Symbol: CD82
• Entrez:
  • 3732(Human)
• Uniprot:
  • P27701()