CD82/Kai-1 Antibody (423524)

Human whole blood granulocytes were stained with Mouse Anti-Human CD82/Kai‑1 Monoclonal Antibody (Catalog # MAB4616, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 423524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD82/Kai-1 Antibody (423524) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CD82/Kai‑1
  Met1-Tyr267
  Accession # AAH01821
• Specificity: Detects human CD82/Kai‑1. Stains human CD82/Kai‑1 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD82
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD82/Kai-1 Antibody (423524)

• CD82 antigenC33
• CD82 molecule
• CD82
• IA4
• IA4C33 antigen
• Inducible membrane protein R2
• KAI1
• KAI1GR15
• kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4))
• Metastasis suppressor Kangai-1
• R2
• SAR2
• SAR2tetraspanin-27
• ST6
• ST6tspan-27
• Suppressor of tumorigenicity 6 protein
• Tetraspanin-27
• TSPAN27
• Tspan-27
• TSPAN274F9

Background

CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD82/Kai-1 Antibody (MAB4616)(4)

Publications using MAB4616

• Illes, Z;Jørgensen, MM;Bæk, R;Bente, LM;Lauridsen, JT;Hyrlov, KH;Aboo, C;Baumbach, J;Kacprowski, T;Cotton, F;Guttmann, CRG;Stensballe, A; New Enhancing MRI Lesions Associate with IL-17, Neutrophil Degranulation and Integrin Microparticles: Multi-Omics Combined with Frequent MRI in Multiple Sclerosis Biomedicines 2023-11-28 [PMID: 38137391] (Array Development, Human)
• K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers Theriogenology, 2020-04-04;149(0):104-116. 2020-04-04 [PMID: 32259747] (Microarray, Mouse)
• Jorgensen MM, Baek R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 1/1/2015 [PMID: 25862471]
• Li MQ, Hou XF, Shao J, Tang CL, Li DJ The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy. Biol. Reprod., 2010-01-14;82(5):968-79. 2010-01-14 [PMID: 20075392] (Cell-based ELISA, Human)

Bioinformatics

• Gene Symbol: CD82
• Uniprot:
  • AAH01821()