CD82/Kai-1 Antibody (423524) [Allophycocyanin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD82/Kai-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4616A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 423524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CD82/Kai-1 Antibody (423524) [Allophycocyanin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human CD82/Kai‑1
  Met1-Tyr267
  Accession # AAH01821
• Specificity: Detects human CD82/Kai‑1. Stains human CD82/Kai‑1 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD82
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD82/Kai-1 Antibody (423524) [Allophycocyanin]

• CD82 antigenC33
• CD82 molecule
• CD82
• IA4
• IA4C33 antigen
• Inducible membrane protein R2
• KAI1
• KAI1GR15
• kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4))
• Metastasis suppressor Kangai-1
• R2
• SAR2
• SAR2tetraspanin-27
• ST6
• ST6tspan-27
• Suppressor of tumorigenicity 6 protein
• Tetraspanin-27
• TSPAN27
• Tspan-27
• TSPAN274F9

Background

CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD82/Kai-1 Antibody (FAB4616A)(2)

Publications using FAB4616A

• Ning Xie, Sabrina N. Chu, Cassandra B. Schultz, Sunny S. K. Chan Efficient Muscle Regeneration by Human PSC-Derived CD82+ ERBB3+ NGFR+ Skeletal Myogenic Progenitors Cells 1/18/2023 [PMID: 36766703]
• MP Pappas, N Xie, JS Penaloza, SSK Chan Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas Cells, 2022-05-09;11(9):. 2022-05-09 [PMID: 35563894] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD82
• Uniprot:
  • AAH01821()