CD81 Antibody - BSA Free

Western Blot: CD81 Antibody [NBP1-77039] - Analysis in human lung tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking petide.

Immunohistochemistry: CD81 Antibody - BSA Free [NBP1-77039] - Immunohistochemistry of CD81 in human lung tissue with CD81 antibody at 5 u/mL.

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] - Immunofluorescence of CD81 in human brain tissue with CD81 antibody at 10 u/ml.Green: CD81 Antibody Blue: DAPI staining

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] - Immunofluorescence of CD81 in A549 cells with CD81 antibody at 20 ug/mL.

Immunohistochemistry: CD81 Antibody - BSA Free [NBP1-77039] - Immunohistochemistry of CD81 in human brain tissue with CD81 antibody at 2 ug/ml.

Blood plasma sEV from high fertile and low-fertile both confirmed sEV characteristics and differ in total protein concentrations. (A) Total protein content of sEV from low fertile dairy cow plasma are significantly higher than the high fertile. Values are presented as mean +/- SD (**P 0.05; Mann–Whitney test). (E) Spherical shape was confirmed in electron micrographs of exosomes from both (i) HF-EXO and (ii) LF-EXO. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37012302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Hebp1 is delivered by MCP-EVs and contributes to nerve regeneration. (A) Representative transmission electron microscopy (TEM) phase images for detecting isolated MCP-EVs. Scale bar = 100 nm. (B) Representative Western blot for Hebp1, EV positive markers (CD63, CD9 and CD81) or EV negative marker (GM130) in MCPs and MCP-EVs. (C, D) Expression of Hebp1 in MCP-EVs infected with lentivirus expressing shHebp1 or shCon at three doses (1 × 103, 5 × 104, and 1 × 104 TU/ml culture medium) for at least 3 days. (E, F) Densitometric quantification of Hebp1 protein bands using an image analyzer. Results are presented as means +/- SEM (n = 4). (G, H) Immunofluorescence staining for NF in mouse MPG tissues exposed to LPS (2.5 μg/ml) and other indicated conditions for 5 days (G). Lengths of NF-positive neurites in MPG tissues, quantified using an image analyzer (H). Results are presented as means +/- SEM (n = 4). Scale bar, 100 um. (I, J) Migration assays of mouse Schwann cells exposed to LPS (2.5 μg/ml) and other indicated conditions for 24 hours (I). Number of migrated cells in the red dotted rectangle, quantified using an image analyzer (J). Results are presented as means +/- SEM (n = 4; **P < 0.01, ***P < 0.001). The relative ratio of the shCon (control) group was defined as 1. MCPs, mouse cavernous pericytes; EVs, extracellular vesicles; LPS, lipopolysaccharide; MPG, major pelvic ganglion; PBS, phosphate-buffered saline; ns, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37324943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD81 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81. The immunogen is located within amino acids 30 - 80 of CD81.
• Predicted Species: Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD81
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 2.5 ug/ml
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  CD81 Peptide (NBP1-77039PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CD81 Antibody - BSA Free

• CD81 antigen (target of antiproliferative 1)
• CD81 antigen
• CD81 molecule
• CD81
• CVID6
• TAPA1
• TAPA-1
• Target of the antiproliferative 1
• Tetraspanin-28
• TSPAN28
• Tspan-28
• tspan-28,26 kDa cell surface protein TAPA-1
• TSPAN28S5.7

Background

CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, a group of cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that associates with integrins and appears to promote muscle cell fusion and support myotube maintenance. Along with SCARB1, LDL-R, and CLDN1, CD81 has been reported to be an entry factor for the Hepatitis C virus. Finally, recent evidence indicates that the CD81 gene is localized in a tumor-suppressor gene region and is thus a candidate gene for malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD81 Antibody (NBP1-77039)(9)

Publications using NBP1-77039

• Larissa Jank, Ajay Kesharwani, Taekyung Ryu, Deepika Joshi, Dimitrios C. Ladakis, Matthew D. Smith, Saumitra Singh, Tanina Arab, Kenneth W. Witwer, Peter A. Calabresi, Chan-Hyun Na, Pavan Bhargava Characterization of spinal cord tissue-derived extracellular vesicles in neuroinflammation Journal of Neuroinflammation 2024-06-08 [PMID: 38851724]
• Turner N, Abeysinghe P, Sadowski P, Mitchell M Cross-species proteomic and microRNA comparison of extracellular vesicles in human milk, cow�s milk, and infant formula products: moving towards next generation infant formula products bioRxiv 2023-02-24 (Western Blot, Bovine, Human)
• Jiao W, Hao J, Liu JM et al. Mesenchymal stem cells-derived extracellular vesicle-incorporated H19 attenuates cardiac remodeling in rats with heart failure The Kaohsiung journal of medical sciences 2023-10-27 [PMID: 37885317] (Western Blot, Rat)
• Turner N, Abeysinghe P, Flay H et al. SWATH-MS Analysis of Blood Plasma and Circulating Small Extracellular Vesicles Enables Detection of Putative Protein Biomarkers of Fertility in Young and Aged Dairy Cows Journal of proteome research 2023-11-03 [PMID: 37830897]
• Ock J, Wu J, Liu F et al. Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury International Journal of Biological Sciences 2023-05-19 [PMID: 37324943] (EM, Mouse)
• Abeysinghe P, Turner N, Mosaad E et al. Dynamics of inflammatory cytokine expression in bovine endometrial cells exposed to cow blood plasma small extracellular vesicles (sEV) may reflect high fertility Scientific reports 2023-04-03 [PMID: 37012302] (WB, Bovine)
• Li H, Bräunig S, Dhapolar P et al. Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing eLife 2023-03-06 [PMID: 36876630] (IHC-P, Human)
• Yin GN, Shin TY, Ock J et al. Pericyte derived extracellular vesicles mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection International journal of molecular medicine 2022-02-01 [PMID: 34935051] (WB, Mouse)
• Fritsch J, Tchikov V, Hennig L et al. A toolbox for the immunomagnetic purification of signalling organelles Traffic 2018-12-20 [PMID: 30569578] (WB, Human)

Bioinformatics

• Gene Symbol: CD81
• Uniprot:
  • NP_004347()