CD81 Antibody (2664C) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD81 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD81 at approximately 27 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (LNCaP) (NBP3-11687) and SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/ml. A specific band was detected for CD81 at approximately 29 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46152). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

(C) Detection of exosome marker proteins in nanoparticle-enriched fluid after separation by TFF + BIA Strong AEX and Exodus using Wes Protein Simple.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 2664C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD81 Antibody (2664C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: CHO-derived human CD81 protein
  Phe113-Ala201
  Accession # P60033
• Specificity: Detects human CD81 in Elisas.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 4 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD81 Antibody (2664C) [Unconjugated]

• CD81 antigen (target of antiproliferative 1)
• CD81 antigen
• CD81 molecule
• CD81
• CVID6
• TAPA1
• TAPA-1
• Target of the antiproliferative 1
• Tetraspanin-28
• TSPAN28
• Tspan-28
• tspan-28,26 kDa cell surface protein TAPA-1
• TSPAN28S5.7

Background

CD81, also known as TAPA-1 and Tetraspanin-28, is an approximately 25 kDa palmitoylated component of plasma membrane lipid rafts (1). It contains four transmembrane segments, two extracellular loops of 30 and 90 amino acids (aa), and three short cytoplasmic regions (2, 3). Within the large extracellular loop, human CD81 shares 90% and 84% aa sequence identity with mouse and rat CD81, respectively. CD81 associates with a wide range of membrane proteins including CD151, TfR2, LDL R, PCSK9, Glypican 3, IFITM1, IGSF8/CD316, FPRP, and complexes of CD19-CD21 (4-11). It is required for the development of CD4+CD8+ DP thymocytes (12) and hepatocyte infection by Plasmodium sporozoites (13). It also supports B cell receptor signaling (11), Hepcidin expression (5), monocyte and B cell tethering to the vascular endothelium (14), and the immunosuppressive function of Treg and MDSC (15). CD81 additionally functions as a receptor for the E2 glycoprotein of hepatitis C virus (16). The CD81-E2 interaction inhibits NK cell cytolytic activity, provides a co-stimulatory signal to T cells, and inhibits the maturation of plasmacytoid dendritic cells (17-19).

1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
2. Oren, R. et al. (1990) Mol. Cell. Biol. 10:4007.
3. Levy, S. et al. (1991) J. Biol. Chem. 266:14597.
4. Zhu, Y.-Z. et al. (2012) Virology 429:112.
5. Chen, J. and C.A. Enns (2015) J. Biol. Chem. 290:7841.
6. Le, Q.-T. et al. (2015) J. Biol. Chem. 290:23385.
7. Takahashi, S. et al. (1990) J. Immunol. 145:2207.
8. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
9. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:4853.
10. Liu, B. et al. (2009) Am. J. Pathol. 175:717.
11. Cherukuri, A. et al. (2004) J. Immunol. 279:31973.
12. Boismenu, R. et al. (1996) Science 271:198.
13. Silvie, O. et al. (2003) Nat. Med. 9:93.
14. Feigelson, S.W. et al. (2003) J. Biol. Chem. 278:51203.
15. Vences-Catalan, F. et al. (2015) Cancer Res. 75:4517.
16. Pileri, P. et al. (1998) Science 282:938.
17. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
18. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
19. Tu, Z. et al. (2013) Cell. Immunol. 284:98.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P60033()