CD81 Antibody (2664C) [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clone: 2664C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

CD81 Antibody (2664C) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human CD81 in Elisas.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD81 Antibody (2664C) [Alexa Fluor™ Plus 488]

• CD81 antigen (target of antiproliferative 1)
• CD81 antigen
• CD81 molecule
• CD81
• CVID6
• TAPA1
• TAPA-1
• Target of the antiproliferative 1
• Tetraspanin-28
• TSPAN28
• Tspan-28
• tspan-28,26 kDa cell surface protein TAPA-1
• TSPAN28S5.7

Background

CD81, also known as TAPA-1 and Tetraspanin-28, is an approximately 25 kDa palmitoylated component of plasma membrane lipid rafts (1). It contains four transmembrane segments, two extracellular loops of 30 and 90 amino acids (aa), and three short cytoplasmic regions (2, 3). Within the large extracellular loop, human CD81 shares 90% and 84% aa sequence identity with mouse and rat CD81, respectively. CD81 associates with a wide range of membrane proteins including CD151, TfR2, LDL R, PCSK9, Glypican 3, IFITM1, IGSF8/CD316, FPRP, and complexes of CD19-CD21 (4-11). It is required for the development of CD4+CD8+ DP thymocytes (12) and hepatocyte infection by Plasmodium sporozoites (13). It also supports B cell receptor signaling (11), Hepcidin expression (5), monocyte and B cell tethering to the vascular endothelium (14), and the immunosuppressive function of Treg and MDSC (15). CD81 additionally functions as a receptor for the E2 glycoprotein of hepatitis C virus (16). The CD81-E2 interaction inhibits NK cell cytolytic activity, provides a co-stimulatory signal to T cells, and inhibits the maturation of plasmacytoid dendritic cells (17-19).

1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
2. Oren, R. et al. (1990) Mol. Cell. Biol. 10:4007.
3. Levy, S. et al. (1991) J. Biol. Chem. 266:14597.
4. Zhu, Y.-Z. et al. (2012) Virology 429:112.
5. Chen, J. and C.A. Enns (2015) J. Biol. Chem. 290:7841.
6. Le, Q.-T. et al. (2015) J. Biol. Chem. 290:23385.
7. Takahashi, S. et al. (1990) J. Immunol. 145:2207.
8. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
9. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:4853.
10. Liu, B. et al. (2009) Am. J. Pathol. 175:717.
11. Cherukuri, A. et al. (2004) J. Immunol. 279:31973.
12. Boismenu, R. et al. (1996) Science 271:198.
13. Silvie, O. et al. (2003) Nat. Med. 9:93.
14. Feigelson, S.W. et al. (2003) J. Biol. Chem. 278:51203.
15. Vences-Catalan, F. et al. (2015) Cancer Res. 75:4517.
16. Pileri, P. et al. (1998) Science 282:938.
17. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
18. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
19. Tu, Z. et al. (2013) Cell. Immunol. 284:98.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.