CD8 Antibody

CD8a was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline CD8a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Fe
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD8 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant feline CD8 alpha
  Ala22-Tyr188
  Accession # P41688
• Specificity: Detects feline CD8 alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CD8 alpha is observed, approximately 5% cross-reactivity with recombinant canine CD8 alpha is observed, and less than 1% cross-reactivity with recombinant human CD8 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD8A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD8 Antibody

• CD_antigen: CD8a
• CD8 antigen, alpha polypeptide (p32)
• CD8
• CD8a molecule
• CD8A
• Leu2 T-lymphocyte antigen
• LEU2
• MAL
• OKT8 T-cell antigen
• p32
• T cell co-receptor
• T8 T-cell antigen
• T-cell antigen Leu2
• T-cell surface glycoprotein CD8 alpha chain
• T-lymphocyte differentiation antigen T8/Leu-2

Background

CD8 is a dimeric complex made up of two Ig superfamily members. The CD8 alpha chain is a 34 kDa type I transmembrane glycoprotein that is disulfide-linked, either to itself forming CD8 alpha alpha , or to an unrelated 30-35 kDa CD8 beta chain, forming CD8 alpha beta . CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR coreceptor, while CD8 alpha alpha promotes T cell survival and differentation. Feline CD8 alpha extracellular region shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha extracellular regions, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD8A
• Uniprot:
  • P41688()