CD63 Antibody (MEM-259) [FITC]

Images

 
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Human HeLa cell line using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained ...read more
Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) Alexa Fluor® 488.
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (cat. ...read more
Immunocytochemistry/ Immunofluorescence: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Human primary fibroblasts using anti-CD63 (MEM-259; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei ...read more
Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 (MEM-259) ...read more
Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PE.
Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.
Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PerCP.
Flow Cytometry: CD63 Antibody (MEM-259) [FITC] [NB500-483] - Analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PE-CyTM5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, IP
Clone
MEM-259
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

CD63 Antibody (MEM-259) [FITC] Summary

Immunogen
HPB-ALL T cell line
Marker
Late Endosomes Marker
Specificity
The antibody MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T-cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD63
Purity
Size Exclusion Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD63 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS and 0.2% (w/v) high grade BSA (protease free)
Preservative
15mM Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for CD63 Antibody (MEM-259) [FITC]

  • CD63 antigen (melanoma 1 antigen)
  • CD63 antigen
  • CD63 molecule
  • CD63
  • Granulophysin
  • Lamp-3
  • Lysosomal-associated membrane protein 3
  • ME491
  • melanoma 1 antigen
  • Melanoma-associated antigen ME491
  • melanoma-associated antigen MLA1
  • MLA1lysosome-associated membrane glycoprotein 3
  • Ocular melanoma-associated antigen
  • OMA81H
  • tetraspanin-30
  • Tspan30
  • tspan-30
  • TSPAN30granulophysin

Background

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD63 Antibody (NB500-483) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-77913AF350
Alexa Fluor 405 NB100-77913AF405
Alexa Fluor 488 NB100-77913AF488
Alexa Fluor 532 NB100-77913AF532
Alexa Fluor 594 NB100-77913AF594
Alexa Fluor 647 NB100-77913AF647
Alexa Fluor 700 NB100-77913AF700
Alexa Fluor 750 NB100-77913AF750
Allophycocyanin NB100-77913APC
Allophycocyanin/Cy7 NB100-77913APCCY7
DyLight 350 NB100-77913UV
DyLight 405 NB100-77913V
DyLight 488 NB100-77913G
DyLight 550 NB100-77913R
DyLight 594 NB100-77913DL594
DyLight 650 NB100-77913C
DyLight 680 NB100-77913FR
DyLight 755 NB100-77913IR
FITC NB500-483
HRP NB100-77913H
Janelia Fluor 549 NB100-77913JF549
Janelia Fluor 646 NB100-77913JF646
PE NB100-77913PE
PE/Atto594 NB100-77913PEATT594
PE/Cy5.5 NB100-77913PECY55
PE/Cy7 NB100-77913PECY7
PerCP NB100-77913PCP

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Tools for Isolation, Quantification and Analysis of Exosomes
Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

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CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

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Bioinformatics

Gene Symbol CD63