CD63 Antibody (7G4.2E8)

Images

 
Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.
Flow Cytometry: CD63 Antibody (7G4.2E8) [NBP2-36567] - Intracellular staining of mouse CD63 in Flow cytometry using 2.0 ug of antibody per 1 million cells. Isotype control was rat IgG2a lambda.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC
Clone
7G4.2E8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD63 Antibody (7G4.2E8) Summary

Immunogen
Partial recombinant mouse CD63 protein (between amino acids 100-250) [UniProt P41731]
Isotype
IgG2a Lambda
Clonality
Monoclonal
Host
Rat
Gene
CD63
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/ml
  • Flow Cytometry 2 ug / 1 million cells
  • Flow (Intracellular) 2 ug / 1 million cells
Publications
Read Publication using
NBP2-36567 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD63 Antibody (7G4.2E8)

  • CD_antigen: CD63
  • CD63 antigen (melanoma 1 antigen)
  • CD63 antigen
  • CD63 molecule
  • CD63
  • Granulophysin
  • Lamp-3
  • Lysosomal-associated membrane protein 3
  • lysosome-associated membrane glycoprotein 3
  • ME491
  • melanoma 1 antigen
  • Melanoma-associated antigen ME491
  • melanoma-associated antigen MLA1
  • MLA1
  • Ocular melanoma-associated antigen
  • OMA81H
  • tetraspanin-30
  • Tspan30
  • tspan-30

Background

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD63 Antibody (NBP2-36567)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD63 Antibody (NBP2-36567) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-36567AF350
Alexa Fluor 405 NBP2-36567AF405
Alexa Fluor 488 NBP2-36567AF488
Alexa Fluor 532 NBP2-36567AF532
Alexa Fluor 594 NBP2-36567AF594
Alexa Fluor 647 NBP2-36567AF647
Alexa Fluor 700 NBP2-36567AF700
Alexa Fluor 750 NBP2-36567AF750
Allophycocyanin NBP2-36567APC
Biotin NBP2-36567B
DyLight 350 NBP2-36567UV
DyLight 405 NBP2-36567V
DyLight 488 NBP2-36567G
DyLight 550 NBP2-36567R
DyLight 594 NBP2-36567DL594
DyLight 650 NBP2-36567C
DyLight 680 NBP2-36567FR
DyLight 755 NBP2-36567IR
HRP NBP2-36567H
Janelia Fluor 549 NBP2-36567JF549
Janelia Fluor 646 NBP2-36567JF646
PE NBP2-36567PE
PerCP NBP2-36567PCP

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Blogs on CD63.

Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma
By Jamshed Arslan, Pharm. D., PhD. Gastroesophageal adenocarcinoma originates from the glandular epithelium of the esophagus, gastroesophageal junction and stomach. The incidence of gastroesophageal adenocarcinoma is...  Read full blog post.

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Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

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Bioinformatics

Gene Symbol CD63
Entrez
Uniprot