CD63 Antibody (7G4.2E8)

Images

 
Western Blot: CD63 Antibody (7G4.2E8) [NBP2-36567] - Western blot analysis of mouse CD63 on recombinant mouse CD63 and mouse kidney lysate.
Flow Cytometry: CD63 Antibody (7G4.2E8) [NBP2-36567] - Intracellular staining of mouse CD63 in Flow cytometry using 2.0 ug of antibody per 1 million cells. Isotype control was rat IgG2a lambda.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC
Clone
7G4.2E8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD63 Antibody (7G4.2E8) Summary

Immunogen
Partial recombinant mouse CD63 protein (between amino acids 100-250) [UniProt P41731]
Isotype
IgG2a Lambda
Clonality
Monoclonal
Host
Rat
Gene
CD63
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/ml
  • Flow Cytometry 2 ug / 1 million cells
  • Flow (Intracellular) 2 ug / 1 million cells
Publications
Read Publication using
NBP2-36567 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD63 Antibody (7G4.2E8)

  • CD63 antigen (melanoma 1 antigen)
  • CD63 antigen
  • CD63 molecule
  • CD63
  • Granulophysin
  • Lamp-3
  • Lysosomal-associated membrane protein 3
  • ME491
  • melanoma 1 antigen
  • Melanoma-associated antigen ME491
  • melanoma-associated antigen MLA1
  • MLA1lysosome-associated membrane glycoprotein 3
  • Ocular melanoma-associated antigen
  • OMA81H
  • tetraspanin-30
  • Tspan30
  • tspan-30
  • TSPAN30granulophysin

Background

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD63 Antibody (NBP2-36567)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD63 Antibody (NBP2-36567) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-36567AF405
Alexa Fluor 488 Labeled NBP2-36567AF488
Alexa Fluor 647 Labeled NBP2-36567AF647
Alexa Fluor 700 Labeled NBP2-36567AF700
Allophycocyanin Labeled NBP2-36567APC
Biotin Labeled NBP2-36567B
DyLight 350 Labeled NBP2-36567UV
DyLight 405 Labeled NBP2-36567V
DyLight 488 Labeled NBP2-36567G
DyLight 550 Labeled NBP2-36567R
DyLight 650 Labeled NBP2-36567C
DyLight 680 Labeled NBP2-36567FR
DyLight 755 Labeled NBP2-36567IR
HRP Labeled NBP2-36567H
PE Labeled NBP2-36567PE
PerCP Labeled NBP2-36567PCP

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Bioinformatics

Gene Symbol CD63
Entrez
Uniprot