CD58/LFA-3 Antibody

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD58/LFA-3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD58/LFA‑3
  Phe29-Arg215
  Accession # P19256
• Specificity: Detects human CD58/LFA‑3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD58
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 3-12 ug/mL
• Control:

  CD58/LFA-3 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD58/LFA-3 Antibody

• Ag3
• CD58 antigen
• CD58 antigen, (lymphocyte function-associated antigen 3)
• CD58 molecule
• CD58
• FLJ23181
• FLJ43722
• LFA3
• LFA-3
• LFA3ag3
• lymphocyte function-associated antigen 3
• Surface glycoprotein LFA-3

Background

T cells require a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), is a 210 amino acid protein that belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production, and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58, and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4).

1. Davis, S.J. and P.A. van der Merwe (1996) Immunol. Today 17:177.
2. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
3. Bolhuis, R.L. Roozemond, R.C. and R.J. van de Griend (1986) J. Immunol. 136:3939.
4. Crawford, K. et al. (2003) Blood 102:1745.
5. Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
6. Bullens, M.A. et al. (2001) International Immunol. 12:181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD58/LFA-3 Antibody (AF1689)(1)

Publication using AF1689

• M Kim, SJ Lee, S Shin, KS Park, SY Park, CH Lee Novel natural killer cell-mediated cancer immunotherapeutic activity of anisomycin against hepatocellular carcinoma cells Sci Rep, 2018;8(1):10668. 2018 [PMID: 30006566] (Neutralization, Human)

Bioinformatics

• Gene Symbol: CD58
• Uniprot:
  • P19256()