CD58/LFA-3 Antibody

CD58/LFA‑3 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human CD58/LFA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes in lymphatic nodules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human CD58/LFA‑3 (Catalog # 1689‑CD) stimulates proliferation in the human CD3+ T cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CD58/LFA‑3 (0.2 µg/well) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD58/LFA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689). The ND50 is typically 3-12 µg/mL in the presence of Mouse Anti-Human CD3 Monoclonal Antibody OKT3 (2 ng/well).

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD58/LFA-3 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD58/LFA‑3
  Phe29-Arg215
  Accession # P19256
• Specificity: Detects human CD58/LFA‑3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD58
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 3-12 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD58/LFA-3 Antibody

• Ag3
• CD58 antigen
• CD58 antigen, (lymphocyte function-associated antigen 3)
• CD58 molecule
• CD58
• FLJ23181
• FLJ43722
• LFA3
• LFA-3
• LFA3ag3
• lymphocyte function-associated antigen 3
• Surface glycoprotein LFA-3

Background

T cells require a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), is a 210 amino acid protein that belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production, and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58, and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4).

1. Davis, S.J. and P.A. van der Merwe (1996) Immunol. Today 17:177.
2. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
3. Bolhuis, R.L. Roozemond, R.C. and R.J. van de Griend (1986) J. Immunol. 136:3939.
4. Crawford, K. et al. (2003) Blood 102:1745.
5. Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
6. Bullens, M.A. et al. (2001) International Immunol. 12:181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD58/LFA-3 Antibody (AF1689)(3)

Publications using AF1689

• Y Zhang, Q Liu, J Liu, Q Liao Upregulated CD58 is associated with clinicopathological characteristics and poor prognosis of patients with pancreatic ductal adenocarcinoma Cancer Cell International, 2021-06-30;21(1):327. 2021-06-30 [PMID: 34193136] (IHC, Human)
• M Kim, SJ Lee, S Shin, KS Park, SY Park, CH Lee Novel natural killer cell-mediated cancer immunotherapeutic activity of anisomycin against hepatocellular carcinoma cells Sci Rep, 2018-07-13;8(1):10668. 2018-07-13 [PMID: 30006566] (Neutralization, Human)
• Madhavi Challa-Malladi, Yen K. Lieu, Olivia Califano, Antony B. Holmes, Govind Bhagat, Vundavalli V. Murty et al. Combined Genetic Inactivation of Beta2-Microglobulin and CD58 Reveals Frequent Escape from Immune Recognition in Diffuse Large B-cell Lymphoma Cancer Cell 2011-12-13 [PMID: 22137796]

Bioinformatics

• Gene Symbol: CD58
• Uniprot:
  • P19256()