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CD53 Antibody (004)

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Western Blot: CD53 Antibody (004) [NBP2-90697] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CD53 Antibody (004) and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-Mouse Mouse CD53 Antibody (004),at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 24 kDa Observed band size: 24 kDa
Western Blot: CD53 Antibody (004) [NBP2-90697] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CD53 Antibody (004) and 60 ug of Immunomagnetic beads Protein G. Primary ...read more
Western Blot: CD53 Antibody (004) [NBP2-90697] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clone
004
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-90697AF350
Alexa Fluor 405
NBP2-90697AF405
Alexa Fluor 488
NBP2-90697AF488
Alexa Fluor 532
NBP2-90697AF532
Alexa Fluor 594
NBP2-90697AF594
Alexa Fluor 647
NBP2-90697AF647
Alexa Fluor 700
NBP2-90697AF700
Alexa Fluor 750
NBP2-90697AF750
Biotin
NBP2-90697B
DyLight 350
NBP2-90697UV
DyLight 405
NBP2-90697V
DyLight 488
NBP2-90697G
DyLight 550
NBP2-90697R
DyLight 594
NBP2-90697DL594
DyLight 650
NBP2-90697C
DyLight 680
NBP2-90697FR
DyLight 755
NBP2-90697IR
HRP
NBP2-90697H
Janelia Fluor 549
NBP2-90697JF549
Janelia Fluor 646
NBP2-90697JF646
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CD53 Antibody (004) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD53 (Q61451; Glu107-Asn181).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD53
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 0.5-2 ul/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for CD53 Antibody (004)

  • antigen MOX44 identified by monoclonal MRC-OX44
  • CD53 antigentetraspanin-25
  • CD53 glycoprotein
  • CD53 molecule
  • CD53 tetraspan antigen
  • CD53
  • cell surface antigen
  • Cell surface glycoprotein CD53
  • MOX44
  • MOX44transmembrane glycoprotein
  • Tetraspanin-25
  • TSPAN25
  • tspan-25
  • TSPAN25leukocyte surface antigen CD53

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-79843
Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).
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Flow (Intracellular): CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HepG2 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - The same samples and volumes were run under reducing and non-reducing conditions. All procedures were performed in parallel for both conditions. L- ladder +- exosomes. Image visualized with HRP linked to secondary antibody. Western blot image submitted by a verified customer review.
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NBP2-21792
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[Unconjugated]
210-TA
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Bioinformatics

Gene Symbol CD53