CD53 Antibodies
We offer CD53 Antibodies for use in common research applications: Flow Cytometry, Functional, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each CD53 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CD53 Antibodies can be used in a variety of model species: Baboon (Negative), Human, Mouse, Rat. Use the list below to choose the CD53 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CD53 polyclonal antibodies and browse our CD53 monoclonal antibody catalog.
Alternate Names for CD53 Antibodies
anti-CD53 antibody, anti-antigen MOX44 identified by monoclonal MRC-OX44 antibody, anti-CD53 antigentetraspanin-25 antibody, anti-CD53 glycoprotein antibody, anti-CD53 molecule antibody, anti-CD53 tetraspan antigen antibody, anti-cell surface antigen antibody, anti-Cell surface glycoprotein CD53 antibody, anti-MOX44transmembrane glycoprotein antibody, anti-Tetraspanin-25 antibody, anti-Tspan-25 antibody, anti-TSPAN25leukocyte surface antigen CD53 antibody
22 Results for "cd53" in Primary Antibodies
![Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.](http://images.novusbio.com/fullsize/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0002.jpg "Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.")
![Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0001.jpg)
![Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD53-Antibody-HD77-Chimeric-Western-Blot-NBP3-12059-img0001.jpg "Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12059 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12059, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12059_rabbit-monoclonal-cd53-antibody-hd77-chimeric-74202522265216.png)
![Flow Cytometry: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12059 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12059_rabbit-monoclonal-cd53-antibody-hd77-chimeric-7420252214126.png)
![Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-278202416331213.jpg "Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD53 Antibody (JE60-38) [NBP3-32159] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD53 antibody (NBP3-32159) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32159) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-298202414333654.jpg)
![Immunocytochemistry/ Immunofluorescence: CD53 Antibody (JE60-38) [NBP3-32159] - Immunocytochemistry analysis of Jurkat cells labeling CD53 with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-29202410334023.jpg)
![Western Blot: CD53 Antibody (SR1376) [NBP3-21921] - Western blot analysis using NBP3-21921 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21921_rabbit-cd53-mab-sr1376-6720239533737.jpg "Western Blot: CD53 Antibody (SR1376) [NBP3-21921] - Western blot analysis using NBP3-21921 in Jurkat cell lysate.")
![Western Blot: CD53 Antibody (104) [NBP2-90952] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.](http://images.novusbio.com/fullsize/CD53-Antibody-104-Western-Blot-NBP2-90952-img0004.jpg "Western Blot: CD53 Antibody (104) [NBP2-90952] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.")
![Immunoprecipitation: CD53 Antibody (104) [NBP2-90952] - CD53 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate. Lane B:0.5 mg Raji Whole Cell Lysate. 2 uL anti-CD53 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-cyno CD53 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.](http://images.novusbio.com/fullsize/CD53-Antibody-104-Immunoprecipitation-NBP2-90952-img0003.jpg)
![Western Blot: CD53 Antibody (004) [NBP2-90697] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CD53 Antibody (004) and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-Mouse Mouse CD53 Antibody (004),at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 24 kDa Observed band size: 24 kDa](http://images.novusbio.com/fullsize/CD53-Antibody-004-Western-Blot-NBP2-90697-img0003.jpg "Western Blot: CD53 Antibody (004) [NBP2-90697] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CD53 Antibody (004) and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-Mouse Mouse CD53 Antibody (004),at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 24 kDa Observed band size: 24 kDa")
![Western Blot: CD53 Antibody (004) [NBP2-90697] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/CD53-Antibody-004-Western-Blot-NBP2-90697-img0002.jpg)
![Immunohistochemistry-Paraffin: CD53 Antibody [NBP2-14464] Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD53-Antibody-Immunohistochemistry-Paraffin-NBP2-14464-img0001.jpg "Immunohistochemistry-Paraffin: CD53 Antibody [NBP2-14464] Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.")