CD45RO Antibody (SPM125) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
SPM125
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

CD45RO Antibody (SPM125) [Janelia Fluor® 646] Summary

Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Localization
Cell surface
Marker
T-Cell Marker
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Chimpanzee

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD45RO Antibody (SPM125) [Janelia Fluor® 646]

  • B220
  • CD45 antigen
  • CD45R
  • CD45RO
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD45RO Antibody (NBP2-34735JF646). (Showing 1 - 1 of 1 FAQ).

  1. I cannot find any anti-mouse CD45RO for FACS. Have any of your anti-Human CD45RO been tested on mouse T cells?
    • Unfortunately, none of our CD45RO antibodies for Flow Cytometry have been validated on mouse. If you would be interested in testing the antibody on mouse, we would recommend Our Innovators Reward Program. Under the terms and conditions of this program we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review including an image of either positive or negative staining for testing in a new species and/or application. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34735AF350
Alexa Fluor 405 NBP2-34735AF405
Alexa Fluor 488 NBP2-34735AF488
Alexa Fluor 532 NBP2-34735AF532
Alexa Fluor 594 NBP2-34735AF594
Alexa Fluor 647 NBP2-34735AF647
Alexa Fluor 700 NBP2-34735AF700
Alexa Fluor 750 NBP2-34735AF750
Allophycocyanin NBP2-34735APC
Biotin NBP2-34735B
DyLight 350 NBP2-34735UV
DyLight 405 NBP2-34735V
DyLight 488 NBP2-34735G
DyLight 550 NBP2-34735R
DyLight 594 NBP2-34735DL594
DyLight 650 NBP2-34735C
DyLight 680 NBP2-34735FR
DyLight 755 NBP2-34735IR
FITC NBP2-34735F
HRP NBP2-34735H
Janelia Fluor 549 NBP2-34735JF549
Janelia Fluor 646 NBP2-34735JF646
PE NBP2-34735PE
PerCP NBP2-34735PCP

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Bioinformatics Tool for CD45RO Antibody (NBP2-34735JF646)

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Bioinformatics

Gene Symbol PTPRC