CD45RA Antibody (PTPRC/7018R)

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA antibody (PTPRC/7018R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA antibody (PTPRC/7018R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: PTPRC/7018R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD45RA Antibody (PTPRC/7018R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14160)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
  
  Theoretical molecular weight: 180-220kDa
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human CD45RA protein (Uniprot: P08575)
• Localization: Cell surface and cytoplasmic
• Marker: B-Cell Marker
• Specificity: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PTPRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 0.5-1.0 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD45RA Antibody (PTPRC/7018R)

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

The CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC
• Uniprot:
  • P08575()
  • P08575()