CD45RA Antibody (158-4D3) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
158-4D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

CD45RA Antibody (158-4D3) - Azide and BSA Free Summary

Immunogen
Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.
Localization
Cell surface
Marker
Leucocyte Marker
Specificity
Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • CyTOF-ready
Application Notes
The Workshop V code for clone 158-4D3 is CD45.38.
Flow Cytometry and Immunohistochemistry-Paraffin applications cited in literature.

Reactivity Notes

Chimpanzee, Common Marmoset

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD45RA Antibody (158-4D3) - Azide and BSA Free

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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CD45RA NBP2-33144

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Bioinformatics

Gene Symbol PTPRC