CD45RA Antibody (158-4D3)


Immunohistochemistry-Paraffin: CD45RA Antibody (158-4D3) [NBP2-15193] - Formalin-paraffin human tonsil stained with CD45RO Ab (UCHL-1).
Flow Cytometry: CD45RA Antibody (158-4D3) [NBP2-15193] - Surface staining of 10^6 PBMC using 1 ug of CD45RA antibody (red), anti mouse IgG FITC secondary and mouse IgG2a isotype control (green).

Product Details

Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

CD45RA Antibody (158-4D3) Summary

Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.
Cell surface
Leucocyte Marker
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
IgG2a Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS with 0.05% BSA.
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein G purified


Application Notes
Human Chromosome Location: 1q31.3
Mol. Weight of Antigen: 205-220kDa
The Workshop V code for clone 158-4D3 is CD45.38.
Flow Cytometry and Immunohistochemistry-Paraffin applications cited in literature.
Read Publication using NBP2-15193.

Reactivity Notes

Chimpanzee, Common Marmoset

Alternate Names for CD45RA Antibody (158-4D3)

  • B220
  • CD45 antigen
  • CD45R
  • EC
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


CD45 (leukocyte common antigen), the most common hematopoietic lineage marker, is a transmembrane glycoprotein tyrosine phosphatase with important roles in immune system signal transduction pathways. Antibody panels have been instrumental in identifying multiple isoforms, including CD45RA. Isoforms differ in their extracellular domain and are generated by alternative splicing of exons 4, 5 and 6 encoding peptide segments called A, B, and C, respectively. At least five different isoforms have been identified in humans: containing all three (ABC), two (AB and BC), one (B) or no (O) exons. A given antibody recognizes either every isoform (CD45 antibody) or only a subset (restricted CD45R antibody). The CD45RA 158-4D3 antibody clone reacts with the ABC and BC isoforms (Schlossman et al, 1995). CD45RA has a molecular weight of 205-220 kDa.
Isoform expression is regulated in lymphocyte type and activation-state dependent manners. CD45RA is expressed on subsets of T and B cells rendering the CD45RA antibody particularly useful for studying subpopulations. For example, naive T lymphocytes express CD45RA containing the A exon which is lost after activation and replaced by CD45R0. In this regard, the CD45RA antibody is a useful naive T cells marker since they express CD45RA, and activated or memory T cells are CD45RA negative and CD45RO positive (Schlossman et al, 1995). CD45RA is differentially expressed on lymphomas and the CD45RA antibody may also be used to help identify or classify lymphomas such as the CD45RA positive B from the CD45R0 positive T cell lymphomas. Researchers are encouraged to consult the scientific literature for additional information about CD45RA expression, including the use of antibody panels that include a CD45RA antibody for characterizing a given malignancy.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD45RA Antibody (NBP2-15193)(1)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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