CD45RA Antibody (158-4D3)

Images

 
Immunohistochemistry-Paraffin: CD45RA Antibody (158-4D3) [NBP2-15193] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (158-4D3).
Flow Cytometry: CD45RA Antibody (158-4D3) [NBP2-15193] - Surface staining of 10^6 PBMC using 1 ug of CD45RA antibody (red), anti mouse IgG FITC secondary and mouse IgG2a isotype control (green).

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
158-4D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD45RA Antibody (158-4D3) Summary

Immunogen
Stimulated human leukocytes were used as immunogen to generate the CD45RA antibody.
Localization
Cell surface
Marker
Leucocyte Marker
Specificity
Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
Application Notes
Human Chromosome Location: 1q31.3
Mol. Weight of Antigen: 205-220kDa
The Workshop V code for clone 158-4D3 is CD45.38.
Flow Cytometry and Immunohistochemistry-Paraffin applications cited in literature.
Publications
Read Publication using NBP2-15193.

Reactivity Notes

Chimpanzee, Common Marmoset

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for CD45RA Antibody (158-4D3)

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45 (leukocyte common antigen), the most common hematopoietic lineage marker, is a transmembrane glycoprotein tyrosine phosphatase with important roles in immune system signal transduction pathways. Antibody panels have been instrumental in identifying multiple isoforms, including CD45RA. Isoforms differ in their extracellular domain and are generated by alternative splicing of exons 4, 5 and 6 encoding peptide segments called A, B, and C, respectively. At least five different isoforms have been identified in humans: containing all three (ABC), two (AB and BC), one (B) or no (O) exons. A given antibody recognizes either every isoform (CD45 antibody) or only a subset (restricted CD45R antibody). The CD45RA 158-4D3 antibody clone reacts with the ABC and BC isoforms (Schlossman et al, 1995). CD45RA has a molecular weight of 205-220 kDa.
Isoform expression is regulated in lymphocyte type and activation-state dependent manners. CD45RA is expressed on subsets of T and B cells rendering the CD45RA antibody particularly useful for studying subpopulations. For example, naive T lymphocytes express CD45RA containing the A exon which is lost after activation and replaced by CD45R0. In this regard, the CD45RA antibody is a useful naive T cells marker since they express CD45RA, and activated or memory T cells are CD45RA negative and CD45RO positive (Schlossman et al, 1995). CD45RA is differentially expressed on lymphomas and the CD45RA antibody may also be used to help identify or classify lymphomas such as the CD45RA positive B from the CD45R0 positive T cell lymphomas. Researchers are encouraged to consult the scientific literature for additional information about CD45RA expression, including the use of antibody panels that include a CD45RA antibody for characterizing a given malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PTPRC
Uniprot