CD45 Antibody (LT40) [FITC]


Flow Cytometry: CD45 Antibody (LT40) [FITC] [NBP1-28296] - Chicken thymocytes were double-stained with R-PElabeled mouse anti-chicken CD45 and mouse antichicken CD3-FITC. Small lymphocytes were then gated and analyzed more

Product Details

Reactivity ChSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

CD45 Antibody (LT40) [FITC] Summary

Chicken CD45 (Mr 190 to 215-kDa).
Chicken CD45 (Mr 190 to 215-kDa).
IgM Kappa
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C in the dark.
FITC (A=495, E=519)
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
IgM purified


Application Notes
Immunoprecipitation, Studies of avian T cell development.

Reactivity Notes



Protect conjugated forms from light.

Alternate Names for CD45 Antibody (LT40) [FITC]

  • B220
  • CD45 antigen
  • CD45
  • CD45R
  • EC
  • LCA
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 Glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. (1) Levels of CD45 expression detected by MAb LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45lo) of thymocytes. The CD45lo population contains exclusively thymocytes with the CD3-CD4-CD8lo phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8lo to CD4+CD8+ and before the expression of CD3. The ability of MAb LT40 to discriminate this CD45lo subpopulation provides a suitable marker for the CD3-CD4-CD8lo immediate precursors to the CD3-CD4+CD8+ thymocytes. (1)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow, Func, In vitro, In vivo, Flow-CS
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu, Mu
Applications: WB, Flow
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow
Species: Hu
Applications: WB, IHC, IHC-P
Species: Mu
Applications: WB, Flow, IHC
Species: Ch
Applications: Flow, IHC, IHC-Fr, IHC-P, IP

Publications for CD45 Antibody (NBP1-28296) (0)

There are no publications for CD45 Antibody (NBP1-28296).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD45 Antibody (NBP1-28296) (0)

There are no reviews for CD45 Antibody (NBP1-28296). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD45 Antibody (NBP1-28296). (Showing 1 - 2 of 2 FAQ).

  1. We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
  2. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.

Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28297
PE Labeled NBP1-28298

Additional CD45 Antibody (LT40) [FITC] Products

Related Products by Gene

Bioinformatics Tool for CD45 Antibody (NBP1-28296)

Discover related pathways, diseases and genes to CD45 Antibody (NBP1-28296). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD45 Antibody (NBP1-28296)

Discover more about diseases related to CD45 Antibody (NBP1-28296).

Pathways for CD45 Antibody (NBP1-28296)

View related products by pathway.

PTMs for CD45 Antibody (NBP1-28296)

Learn more about PTMs related to CD45 Antibody (NBP1-28296).

Research Areas for CD45 Antibody (NBP1-28296)

Find related products by research area.

Blogs on CD45.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PTPRC

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-28296 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

CD45 Recombinant Protein
CD45 Recombinant Protein