CD44 Antibody (OTI1E3) - Azide and BSA Free

Western Blot: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD44.

Immunocytochemistry/Immunofluorescence: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD44.

Immunohistochemistry: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of Human pancreas tissue.

Flow Cytometry: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of Color205 cells, using CD44 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of Carcinoma of Human lung tissue.

Immunohistochemistry: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of Carcinoma of Human pancreas tissue.

Immunohistochemistry: CD44 Antibody (OTI1E3) - Azide and BSA Free [NBP2-70379] - Analysis of Human breast tissue.

Product Discontinued

CD44 Antibody (OTI1E3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human CD44(NP_000601) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:400-8000
• Theoretical MW: 79.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for CD44 Antibody (OTI1E3) - Azide and BSA Free

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD44