CD44 Antibody (IM7) [FITC]

Flow Cytometry: CD44 Antibody (IM7) [FITC] [NBP1-41266F] - Staining of C57BL/6 splenocytes with 0.25 ug of Rat IgG2b kappa Isotype Control FITC (cat. 11-4031) (open histogram) or 0.25 ug of Anti-Human/Mouse CD44 FITC (filled histogram). Total viable cells were used for analysis.

• Reactivity: Hu, Mu, Ca, Eq, Fe
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: IM7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: FITC

CD44 Antibody (IM7) [FITC] Summary

• Immunogen: Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
• Marker: Cell Membrane Marker
• Specificity: The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CD44
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Cat (100%). Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for CD44 Antibody (IM7) [FITC]

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NBP1-41266F)(1)

Publication using NBP1-41266F

• Wen L, Liu P, Chen Q et al. Impaired Proliferation, Apoptosis, and Angiogenesis of Adipose-Derived Stem Cells Isolated from Rats during the Course of Diabetes Coatings 2021-12-17 (FLOW, Rat)

Review for CD44 Antibody (NBP1-41266F) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: A G Flow Canine 03/24/2017

Summary

• Application: Flow Cytometry
• Sample Tested: Dog whole blood
• Species: Canine
• Lot: 522448-032317-f

Bioinformatics

• Gene Symbol: CD44