CD44 Antibody (IM7) - BSA Free

Western Blot: CD44 Antibody (IM7) [NBP1-41266] - Analysis of CD44 in HeLa cells (positive; lane 2) and MOLT-4 cells (negative; lane 1) using anti-CD44 (IM7) purified; non-reducing conditions.

Flow Cytometry: CD44 Antibody (IM7) [NBP1-41266] - Detection of T-cells in canine whole blood. Image from verified customer review.

Analysis of CD44 in HeLa cells (positive; lane 2) and MOLT-4 cells (negative; lane 1) using anti-CD44 (IM7) purified; non-reducing conditions.

• Reactivity: Hu, Mu, Ca, Eq, Fe
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: IM7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD44 Antibody (IM7) - BSA Free Summary

• Description: Novus Biologicals Rat CD44 Antibody (IM7) - BSA Free (NBP1-41266) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-CD44 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
• Marker: Cell Membrane Marker
• Specificity: The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CD44
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: This antibody is CyTOF ready.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (100%). Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CD44 Antibody (IM7) - BSA Free

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NBP1-41266)(4)

Publications using NBP1-41266

• Varn FS, Johnson KC, Martinek J Et al. Glioma progression is shaped by genetic evolution and microenvironment interactions Cell 2022-06-01 [PMID: 35649412] (IHC-P, Human)
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Haley M, Bere L, Minshull J et al. Hypoxia coordinates the spatial landscape of myeloid cells within glioblastoma to affect outcome bioRxiv 2023-06-30
• Thangadurai M, Sethuraman S, Subramanian A A 2D inflammatory co-culture model for investigating synovial fibroblast and macrophage interactions in rheumatoid arthritis Scientific Reports 2025-08-17 [PMID: 40820240]
• Dogan A, Demirci S, Sahin F. In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells Cell Biol. Int. 2014-07-31 [PMID: 25077982]

Review for CD44 Antibody (NBP1-41266) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer Flow Canine 05/18/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Whole blood cells
• Species: Canine
• Lot: 522448

Comments

• Comments: The whole blood cells were lysed to get rid of the RBC. The washed cell were then incubated with the Antibody. They were washed and then incubated with a conjugated secondary. Allowed to sit for 20mins and then washed again and read on the BD Fortessa.

Bioinformatics

• Gene Symbol: CD44