CD40 Ligand/TNFSF5 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CD40 Ligand/TNFSF5 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CD40 Ligand
  Glu108-Leu261
  Accession # P29965
• Specificity: Detects human CD40 Ligand in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD40LG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD40 Ligand/TNFSF5 Antibody [Biotin]

• CD154 antigen
• CD154
• CD40 antigen ligand
• CD40 Ligand
• CD40L
• CD40-L
• CD40LG
• CD40LIGM
• gp39
• hCD40L
• HIGM1
• T-B cell-activating molecule
• T-BAM
• T-cell antigen Gp39
• TNF-related activation protein
• TNFSF5
• TNFSF5IMD3
• TRAP
• TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
• tumor necrosis factor (ligand) superfamily member 5
• Tumor necrosis factor ligand superfamily member 5

Background

CD40 Ligand is a type II transmembrane glycoprotein belonging to the TNF family (TNFSF5). It is expressed predominantly on activated CD4⁺ T lymphocytes and also on other hematopoietic cells. CD40 L binds to CD40 (TNFRSF5) on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Engagement of CD40 by CD40 Ligand promotes B cell and dendritic cell maturation and activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD40 Ligand/TNFSF5 Antibody (BAF617)(1)

Publication using BAF617

• Lengelle J, Panopoulos E, Betsou F Soluble CD40 ligand as a biomarker for storage-related preanalytic variations of human serum. Cytokine, 2008-10-11;44(2):275-82. 2008-10-11 [PMID: 18851919] (Western Blot, Human)

FAQs for CD40 Ligand/TNFSF5 Antibody (BAF617). (Showing 1 - 1 of 1 FAQ).

1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
   • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD40LG
• Uniprot:
  • P29965()