Western blot shows conditioned media (CM) from Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by ...read more
Western blot shows lysates of Jurkat human acute T cell leukemia cells, Human Platelets and Th1 CD4+. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified ...read more
E. coli-derived recombinant human CD40 Ligand/TNFSF5 Glu108-Leu261 Accession # P29965
Detects human CD40 Ligand/TNFSF5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse CD40 Ligand is observed and less than 1% cross-reactivity with recombinant human (rh) CD27 Ligand and rhCD30 Ligand is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for CD40 Ligand/TNFSF5 Antibody [Unconjugated]
CD40 antigen ligand
T-B cell-activating molecule
T-cell antigen Gp39
TNF-related activation protein
TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
tumor necrosis factor (ligand) superfamily member 5
Tumor necrosis factor ligand superfamily member 5
CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
Armitage, R.J. et al. (1992) Nature 357:80.
Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CD40 Ligand/TNFSF5 Antibody (AF617). (Showing 1 - 1 of 1 FAQs).
I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at email@example.com with any questions regarding this program.
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