CD40 Ligand/TNFSF5 Antibody [Unconjugated]

Western blot shows conditioned media (CM) from Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD40 Ligand/TNFSF5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western blot shows lysates of Jurkat human acute T cell leukemia cells, Human Platelets and Th1 CD4+. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD40 Ligand/TNFSF5 at approximately 17 and 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD40 Ligand/TNFSF5 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CD40 Ligand/TNFSF5
  Glu108-Leu261
  Accession # P29965
• Specificity: Detects human CD40 Ligand/TNFSF5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse CD40 Ligand is observed and less than 1% cross-reactivity with recombinant human (rh) CD27 Ligand and rhCD30 Ligand is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD40LG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD40 Ligand/TNFSF5 Antibody [Unconjugated]

• CD154 antigen
• CD154
• CD40 antigen ligand
• CD40 Ligand
• CD40L
• CD40-L
• CD40LG
• CD40LIGM
• gp39
• hCD40L
• HIGM1
• T-B cell-activating molecule
• T-BAM
• T-cell antigen Gp39
• TNF-related activation protein
• TNFSF5
• TNFSF5IMD3
• TRAP
• TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
• tumor necrosis factor (ligand) superfamily member 5
• Tumor necrosis factor ligand superfamily member 5

Background

CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4⁺ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.

1. Armitage, R.J. et al. (1992) Nature 357:80.
2. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
3. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
4. Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
5. Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD40 Ligand/TNFSF5 Antibody (AF617)(7)

Publications using AF617

• Bollimpelli, VS;Reddy, PBJ;Gangadhara, S;Charles, TP;Burton, SL;Tharp, GK;Styles, TM;Labranche, CC;Smith, JC;Upadhyay, AA;Sahoo, A;Legere, T;Shiferaw, A;Velu, V;Yu, T;Tomai, M;Vasilakos, J;Kasturi, SP;Shaw, GM;Montefiori, D;Bosinger, SE;Kozlowski, PA;Pulendran, B;Derdeyn, CA;Hunter, E;Amara, RR; Intradermal but not intramuscular modified vaccinia Ankara immunizations protect against intravaginal tier2 simian-human immunodeficiency virus challenges in female macaques Nature communications 2023-08-08 [PMID: 37553348] (Flow Cytometry, Human)
• Tiffany M. Styles, Sailaja Gangadhara, Pradeep B. J. Reddy, Anusmita Sahoo, Ayalensh Shiferaw, Sarah Welbourn et al. V2 hotspot optimized MVA vaccine expressing stabilized HIV-1 Clade C envelope Gp140 delays acquisition of heterologous Clade C Tier 2 challenges in Mamu-A*01 negative Rhesus Macaques Frontiers in Immunology 2022-07-22 [PMID: 35935987]
• GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion Redox Biology, 2021-12-05;48(0):102205. 2021-12-05 [PMID: 34891098] (Dot Blot, Human)
• Alireza Labani-Motlagh, Sedigheh Naseri, Jessica Wenthe, Emma Eriksson, Angelica Loskog Systemic immunity upon local oncolytic virotherapy armed with immunostimulatory genes may be supported by tumor-derived exosomes Molecular Therapy - Oncolytics 2021-03-26 [PMID: 33738337]
• Tiffany M. Styles, Sailaja Gangadhara, Pradeep B. J. Reddy, Sakeenah Hicks, Celia C. LaBranche, David C. Montefiori et al. Human Immunodeficiency Virus C.1086 Envelope gp140 Protein Boosts following DNA/Modified Vaccinia Virus Ankara Vaccination Fail To Enhance Heterologous Anti-V1V2 Antibody Response and Protection against Clade C Simian-Human Immunodeficiency Virus Challenge Journal of Virology 2019-10-15 [PMID: 31341049] (Flow Cytometry, Human)
• Liu J, Yu Q, Stone GW, Yue FY, Ngai N, Jones RB, Kornbluth RS, Ostrowski MA CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals. Vaccine, 2008-06-02;26(32):4062-72. 2008-06-02 [PMID: 18562053] (Western Blot, Human)
• Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis. J. Immunol., 2006-05-15;176(10):5735-9. 2006-05-15 [PMID: 16670278] (IHC-Fr, Human)

FAQs for CD40 Ligand/TNFSF5 Antibody (AF617). (Showing 1 - 1 of 1 FAQs).

1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
   • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD40LG
• Uniprot:
  • P29965()