CD40 Ligand/TNFSF5 Antibody (LL2)

Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone

Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone

Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.

• Reactivity: Hu, Ha
• Applications: ELISA, Flow, Func, ICC/IF, B/N
• Clone: LL2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CD40 Ligand/TNFSF5 Antibody (LL2) Summary

• Immunogen: CD40L/CD154 was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD40LG
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000

Reactivity Notes

Human.Hamster reactivity reported in scientific literature (PMID: 25752457)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria, Japan, and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for CD40 Ligand/TNFSF5 Antibody (LL2)

• CD154 antigen
• CD154
• CD40 antigen ligand
• CD40 Ligand
• CD40L
• CD40-L
• CD40LG
• CD40LIGM
• gp39
• hCD40L
• HIGM1
• T-B cell-activating molecule
• T-BAM
• T-cell antigen Gp39
• TNF-related activation protein
• TNFSF5
• TNFSF5IMD3
• TRAP
• TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
• tumor necrosis factor (ligand) superfamily member 5
• Tumor necrosis factor ligand superfamily member 5

Background

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD40 Ligand/TNFSF5 Antibody (DDX0141P-100)(4)

Publications using DDX0141P-100

• Lopez-Herrera G, Maravillas-Montero JL, Vargas-Hernandez A et al. A novel CD40LG deletion causes the hyper-IgM syndrome with normal CD40L expression in a 6-month-old child. Immunol. Res. 2015-03-10 [PMID: 25752457] (FLOW, Hamster)
• Henn V et al. The inflammatory action of CD40 ligand (CD154) expressed on activated human platelets is temporally limited by coexpressed CD40. Blood 2001-08-01 [PMID: 11493450]
• Rissoan MC et al. The functional CD40 antigen of fibroblasts may contribute to the proliferation of rheumatoid synovium. Clin Exp Immunol 1996-12-01 [PMID: 8973616]
• Razanajaona D et al. Somatic mutations in human Ig variable genes correlate with a partially functional CD40-ligand in the X-linked hyper-IgM syndrome. J Immunol 1996-08-01 [PMID: 8759730]

FAQs for CD40 Ligand/TNFSF5 Antibody (DDX0141P-100). (Showing 1 - 1 of 1 FAQs).

1. I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
   • Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD40LG