CD31/PECAM-1 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD31/PECAM-1 at approximately 173-186 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells and sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Expression pattern of integrin receptors and type IV collagen in normal pancreatic tissue. A. H&E staining of normal exocrine pancreas with acini structures and a duct in the insert. The area shown is representative for all panels in B. B. Merged immunofluorescence staining of integrin alpha 1, alpha 2, and beta 1 (in red), and type IV collagen (green in upper row), or the endothelial marker CD31 (green in lower row). Type IV collagen is present in the vascular-, ductal-, and acini-BMs. Integrin alpha 1 is only expressed in endothelial cells and integrin alpha 2 only in the ductal epithelium. Integrin beta 1 is found in the ductal-, endothelial-, and at the base of the acini-cells. Arrows indicate examples of colocalization. Magnification x40 in all panels. Cell nuclei are stained by DAPI (in blue). Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-154), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD31
  Extracellular domain
• Specificity: Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PECAM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Knockout Validated 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD31/PECAM-1 Antibody (AF806)(74)

Publications using AF806

• de Korte, T;Johnson, BB;Kosmidis, G;Samson-Couterie, B;Mol, MPH;van Helden, RWJ;Razaghi, E;François, L;Meraviglia, V;Yiangou, L;Kuipers, T;Mei, H;Bellin, M;Braam, SR;Jain, S;Mummery, CL;Davis, RP; Industrialization of 3D hiPSC-cardiac microtissues for high-throughput cardiac safety and drug discovery screening Trends in biotechnology 2025-12-18 [PMID: 41419348] (Flow Cytometry, Human)
• Fan, L;Tang, Q;Wang, Y;Sun, H;Li, G;Yang, Y;Zhu, H;Liu, Z;Wang, H;Wang, H;Jing, Q;Antos, CL;Xiao, H;Guan, R;Pei, G;Ginhoux, F;Zhou, Z;Zeng, A; Injury-induced Clusterin+ cardiomyocytes suppress inflammation and promote regeneration in neonatal and adult hearts by reprogramming macrophages Cell stem cell 2025-11-07 [PMID: 41205597] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Pan, Z;Yao, Q;Kong, W;Ma, X;Tian, L;Zhao, Y;Zhu, S;Chen, S;Sun, M;Liu, J;Jiang, S;Ma, J;Liu, Q;Peng, X;Li, X;Hong, Z;Hong, Y;Wang, X;Liu, J;Zhang, J;Zhang, W;Sun, B;Pahlavan, S;Xia, Y;Shen, W;Liu, Y;Jiang, W;Xie, Z;Kong, W;Wang, X;Wang, K; Generation of iPSC-derived human venous endothelial cells for the modeling of vascular malformations and drug discovery Cell stem cell 2024-11-22 [PMID: 39579761] (FACS, Mouse)
• Chen, J;Crouch, EE;Zawadzki, ME;Jacobs, KA;Mayo, LN;Choi, JJ;Lin, PY;Shaikh, S;Tsui, J;Gonzalez-Granero, S;Waller, S;Kelekar, A;Kang, G;Valenzuela, EJ;Birrueta, JO;Diafos, LN;Wedderburn-Pugh, K;Di Marco, B;Xia, W;Han, CZ;Coufal, NG;Glass, CK;Fancy, SPJ;Alfonso, J;Kriegstein, AR;Oldham, MC;Garcia-Verdugo, JM;Kutys, ML;Lehtinen, MK;Combes, AJ;Huang, EJ; Proinflammatory immune cells disrupt angiogenesis and promote germinal matrix hemorrhage in prenatal human brain Nature neuroscience 2024-09-30 [PMID: 39349662] (Immunohistochemistry, Human)
• Yao, K;Yang, M;Shu, M;Wang, T;Gao, D;Zhou, L;Wang, G;Zhang, Z;Tang, J; SOX4 promotes vascular abnormality in glioblastoma and is a novel target to improve drug delivery Translational oncology 2024-09-16 [PMID: 39288695] (Immunohistochemistry, Mouse)
• Bhattacharya, R;Ward, T;Kalejaiye, TD;Mishra, A;Leeman, S;Arzaghi, H;Seidman, JG;Seidman, CE;Musah, S; Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39211233] (Immunocytochemistry, Human)
• Yang, L;Han, Y;Zhang, T;Dong, X;Ge, J;Roy, A;Zhu, J;Lu, T;Vandana, JJ;de Silva, N;Robertson, CC;Xiang, JZ;Pan, C;Sun, Y;Que, J;Evans, T;Liu, C;Wang, W;Naji, A;Parker, SCJ;Schwartz, RE;Chen, S; Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic ? cell Pyroptosis upon Viral Infection bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39149298] (Immunocytochemistry, Human)
• Li, B;Xiong, W;Zuo, W;Shi, Y;Wang, T;Chang, L;Wu, Y;Ma, H;Bian, Q;Chang, ACY; Proximal telomeric decompaction due to telomere shortening drives FOXC1-dependent myocardial senescence Nucleic acids research 2024-04-18 [PMID: 38634789] (Immunohistochemistry, Human)
• Zhang, L;Yin, Z;Liu, X;Jin, G;Wang, Y;He, L;Li, M;Pang, X;Yan, B;Jia, Z;Ma, J;Wei, J;Cheng, F;Li, D;Wang, L;Han, Z;Liu, Q;Chen, F;Cao, H;Lei, P; Dietary emulsifier polysorbate 80 exposure accelerates age-related cognitive decline Brain, behavior, and immunity 2024-03-31 [PMID: 38565398] (Immunohistochemistry, Mouse)
• Murdock, MH;Yang, CY;Sun, N;Pao, PC;Blanco-Duque, C;Kahn, MC;Kim, T;Lavoie, NS;Victor, MB;Islam, MR;Galiana, F;Leary, N;Wang, S;Bubnys, A;Ma, E;Akay, LA;Sneve, M;Qian, Y;Lai, C;McCarthy, MM;Kopell, N;Kellis, M;Piatkevich, KD;Boyden, ES;Tsai, LH; Multisensory gamma stimulation promotes glymphatic clearance of amyloid Nature 2024-02-28 [PMID: 38418876] (Immunohistochemistry, Human)
• Raval, AN;Schmuck, EG;Roy, S;Saito, Y;Zhou, T;Conklin, J;Hacker, TA;Koonce, C;Boyer, M;Stack, K;Hebron, E;Nagle, SK;Hsieh, PCH;Kamp, TJ; Human iPSC-derived Committed Cardiac Progenitors Generate Cardiac Tissue Grafts in a Swine Ischemic Cardiomyopathy Model without Triggering Ventricular Arrhythmias bioRxiv : the preprint server for biology 2024-02-17 [PMID: 38405927] (Immunocytochemistry, Human)
• Frenz-Wiessner, S;Fairley, SD;Buser, M;Goek, I;Salewskij, K;Jonsson, G;Illig, D;Zu Putlitz, B;Petersheim, D;Li, Y;Chen, PH;Kalauz, M;Conca, R;Sterr, M;Geuder, J;Mizoguchi, Y;Megens, RTA;Linder, MI;Kotlarz, D;Rudelius, M;Penninger, JM;Marr, C;Klein, C; Generation of complex bone marrow organoids from human induced pluripotent stem cells Nature methods 2024-02-19 [PMID: 38374263] (Immunohistochemistry, Human, Xenograft)
• Shi, G;Liu, L;Cao, Y;Ma, G;Zhu, Y;Xu, J;Zhang, X;Li, T;Mi, L;Jia, H;Zhang, Y;Liu, X;Zhou, Y;Li, S;Yang, G;Liu, X;Chen, F;Wang, B;Deng, Q;Zhang, S;Zhang, J; Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1?/ASK1/JNK signaling pathway in mice with traumatic brain injury Journal of neuroinflammation 2023-09-30 [PMID: 37777772] (IHC, Mouse)
• Y Gu, Y Zhou, S Ju, X Liu, Z Zhang, J Guo, J Gao, J Zang, H Sun, Q Chen, J Wang, J Xu, Y Xu, Y Chen, Y Guo, J Dai, H Ma, C Wang, G Jin, C Li, Y Xia, H Shen, Y Yang, X Guo, Z Hu Multi-omics profiling visualizes dynamics of cardiac development and functions Cell Reports, 2022-12-27;41(13):111891. 2022-12-27 [PMID: 36577384] (Mouse)
• JKE Spetz, MHC Florido, CS Fraser, X Qin, J Choiniere, SJ Yu, R Singh, M Friesen, LL Rubin, JE Salem, JJ Moslehi, KA Sarosiek Heightened apoptotic priming of vascular cells across tissues and life span predisposes them to cancer therapy-induced toxicities Science Advances, 2022-11-09;8(45):eabn6579. 2022-11-09 [PMID: 36351019] (ICC, IHC, Human, Mouse)
• Shin HS, Thakore A, Tada Y et al. Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling Scientific Reports 2022-10-20 [PMID: 36266453]
• Qi Zhang, Chang Liu, Rubing Shi, Shiyi Zhou, Huimin Shan, Lidong Deng et al. Blocking C3d+/GFAP+ A1 Astrocyte Conversion with Semaglutide Attenuates Blood-Brain Barrier Disruption in Mice after Ischemic Stroke Aging and disease 2022-06-01 [PMID: 35656116]
• Kelvin Yin, Daniel Patten, Sarah Gough, Susana de Barros Gonçalves, Adelyne Chan, Ioana Olan et al. Senescence-induced endothelial phenotypes underpin immune-mediated senescence surveillance Genes & Development 2022-05-01 [PMID: 35618311]
• Mai T. Ngo, Victoria R. Barnhouse, Aidan E. Gilchrist, Bhushan P. Mahadik, Christine J. Hunter, Joy N. Hensold et al. Hydrogels Containing Gradients in Vascular Density Reveal Dose‐Dependent Role of Angiocrine Cues on Stem Cell Behavior Advanced Functional Materials 2021-12-16 [PMID: 35558090]
• Lissenya B. Argueta, Lauretta A. Lacko, Yaron Bram, Takuya Tada, Lucia Carrau, André Figueiredo Rendeiro et al. Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy iScience 2022-05-20 [PMID: 35434541] (Immunohistochemistry, Immunocytochemistry, Human)
• Alexandra R. Harris, Savieay Esparza, Mohammad S. Azimi, Robert Cornelison, Francesca N. Azar, Danielle C. Llaneza et al. Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy Frontiers in Oncology 2022-03-17 [PMID: 35372032]
• Kate A. Wickham, Line B. Nørregaard, Mikkel Oxfeldt, Stephen S. Cheung, Lasse Gliemann, Mette Hansen et al. Short-Term Supplementation With Fermented Red Clover Extract Reduces Vascular Inflammation in Early Post-menopausal Women Frontiers in Cardiovascular Medicine 2022-02-10 [PMID: 35224058] (Western Blot, Human)
• Elizabeth S. Taglauer, Elisha M. Wachman, Lillian Juttukonda, Timothy Klouda, Jiwon Kim, Qiong Wang et al. Acute Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Pregnancy Is Associated with Placental Angiotensin-Converting Enzyme 2 Shedding The American Journal of Pathology 2023-04-01 [PMID: 35090860] (Immunohistochemistry, Human)
• Amita Datta-Mannan, Robin Brown, Stephanie Key, Paul Cain, Yiqing Feng Pharmacokinetic Developability and Disposition Profiles of Bispecific Antibodies: A Case Study with Two Molecules Antibodies (Basel) 2021-12-28 [PMID: 35076469]
• G Jo, J Bae, HJ Hong, AR Han, DK Kim, SP Hong, JA Kim, S Lee, GY Koh, HM Kim Structural insights into the clustering and activation of Tie2 receptor mediated by Tie2 agonistic antibody Nature Communications, 2021-11-01;12(1):6287. 11/1/2021 [PMID: 34725372]
• Timothy Klouda, Yuan Hao, Hyunbum Kim, Jiwon Kim, Judith Olejnik, Adam J. Hume et al. Interferon-alpha or -beta facilitates SARS-CoV-2 pulmonary vascular infection by inducing ACE2 Angiogenesis 2022-05-01 [PMID: 34714440]
• Y Roye, R Bhattachar, X Mou, Y Zhou, MA Burt, S Musah A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium Micromachines, 2021-08-16;12(8):. 2021-08-16 [PMID: 34442589] (ICC, Human)
• Lyu Z, Park J, Kim KM Et al. A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke Nature biomedical engineering 8/1/2021 [PMID: 34385693]
• Y Xie, L He, R Lugano, Y Zhang, H Cao, Q He, M Chao, B Liu, Q Cao, J Wang, Y Jiao, Y Hu, L Han, Y Zhang, H Huang, L Uhrbom, C Betsholtz, L Wang, A Dimberg, L Zhang Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing JCI Insight, 2021-08-09;0(0):. 2021-08-09 [PMID: 34228647] (IHC, Human)
• LB Argueta, LA Lacko, Y Bram, T Tada, L Carrau, T Zhang, S Uhl, BC Lubor, V Chandar, C Gil, W Zhang, B Dodson, J Bastiaans, M Prabhu, CM Salvatore, YJ Yang, RN Baergen, BR tenOever, NR Landau, S Chen, RE Schwartz, H Stuhlmann SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta bioRxiv : the preprint server for biology, 2021-06-17;0(0):. 2021-06-17 [PMID: 34100019] (ICC, Human)
• YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS Aging Cell, 2021-06-04;0(0):e13388. 2021-06-04 [PMID: 34086398] (IHC, Western Blot, Human)
• Tang X, Uhl S, Zhang T et al. SARS-CoV-2 infection induces beta cell transdifferentiation Cell Metabolism 5/1/2021 [PMID: 34081913]
• Fabien Kawecki, Todd Galbraith, William P. Clafshenkel, Michel Fortin, François A. Auger, Julie Fradette In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model Materials (Basel) 2021-04-17 [PMID: 33920607] (Immunohistochemistry, Human)
• KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration Cell Stem Cell, 2021-03-29;0(0):. 2021-03-29 [PMID: 33784497] (ICC, Human)
• G Campostrin, V Meraviglia, E Giacomelli, RWJ van Helden, L Yiangou, RP Davis, M Bellin, VV Orlova, CL Mummery Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells Nature Protocols, 2021-03-26;0(0):. 2021-03-26 [PMID: 33772245] (Flow Cytometry, ICC, Human)
• EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development Cell Stem Cell, 2020-09-17;0(0):. 2020-09-17 [PMID: 32946788] (IHC, Human)
• CJ Mietus, TJ Lackner, PS Karvelis, GT Willcockso, CM Shields, NG Lambert, P Koutakis, MA Fuglestad, H Hernandez, GR Haynatzki, JKS Kim, HK DeSpiegela, II Pipinos, GP Casale Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med, 2020-08-08;9(8):. 2020-08-08 [PMID: 32784470] (ICC, IHC, Bovine, Human)
• Abidemi Junaid, Johannes Schoeman, Wei Yang, Wendy Stam, Alireza Mashaghi, Anton Jan van Zonneveld et al. Metabolic response of blood vessels to TNF alpha eLife 2020-08-04 [PMID: 32749215]
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020;27(1):125-136.e7. 2020 [PMID: 32579880] (IHC, Human)
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020-06-19;27(1):125-136.e7. 2020-06-19 [PMID: 32579880] (ICC, IHC, Human)
• C Caneparo, C Baratange, S Chabaud, S Bolduc Conditioned medium produced by fibroblasts cultured in low oxygen pressure allows the formation of highly structured capillary-like networks in fibrin gels Sci Rep, 2020-06-09;10(1):9291. 2020-06-09 [PMID: 32518266] (ICC, Human)
• JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes Nat. Med., 2020-06-08;26(6):952-963. 2020-06-08 [PMID: 32514169] (ICC, Human)
• E Giacomelli, V Meraviglia, G Campostrin, A Cochrane, X Cao, RWJ van Helden, A Krotenberg, M Mircea, S Kostidis, RP Davis, BJ van Meer, CR Jost, AJ Koster, H Mei, DG Míguez, AA Mulder, M Ledesma-Te, G Pompilio, L Sala, DCF Salvatori, RC Slieker, E Sommariva, AAF de Vries, M Giera, S Semrau, LGJ Tertoolen, VV Orlova, M Bellin, CL Mummery Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease Cell Stem Cell, 2020-05-26;26(6):862-879.e11. 2020-05-26 [PMID: 32459996] (IHC, Human)
• Dongdong Zhang, Andrew J. Kinloch, Abhinav Srinath, Robert Shenkar, Romuald Girard, Rhonda Lightle et al. Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu Journal of Autoimmunity 2020-09-01 [PMID: 32362501] (Immunohistochemistry, Human)
• Oleg Varlamov, Matthew Bucher, Leslie Myatt, Natali Newman, Kathleen A. Grant Daily Ethanol Drinking Followed by an Abstinence Period Impairs Bone Marrow Niche and Mitochondrial Function of Hematopoietic Stem/Progenitor Cells in Rhesus Macaques Alcoholism: Clinical and Experimental Research 2020-05-01 [PMID: 32220015]
• Jacob J. Robino, Nathalie Pamir, Sara Rosario, Lindsey B. Crawford, Benjamin J. Burwitz, Charles T. Roberts et al. Spatial and biochemical interactions between bone marrow adipose tissue and hematopoietic stem and progenitor cells in rhesus macaques Bone 2020-04-01 [PMID: 31972314]
• HE Weiss-Bilk, MJ Meagher, JA Gargac, GL Niebur, RK Roeder, DR Wagner Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells Biomater Res, 2019-10-17;23(0):15. 2019-10-17 [PMID: 31641529] (Flourescense Microscopy, Human)
• Alexandra Brahmer, Elmo Neuberger, Leona Esch-Heisser, Nils Haller, Malene Moeller Jorgensen, Rikke Baek et al. Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation Journal of Extracellular Vesicles 2019-12-01 [PMID: 31191831]
• JM Elsakr, JC Dunn, K Tennant, SK Zhao, K Kroeten, RC Pasek, DL Takahashi, TA Dean, DR Velez Edwa, CE McCurdy, KM Aagaard, AC Powers, JE Friedman, P Kievit, M Gannon Maternal Western-style diet affects offspring islet composition and function in a non-human primate model of maternal over-nutrition Mol Metab, 2019-04-12;0(0):. 2019-04-12 [PMID: 31036449] (IHC-P, Primate - Macaca fuscata (Japanese Macaque))
• Gliemann L, Rytter N, Piil P et al. The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle. Front Physiol 12/18/2018 [PMID: 30618819]
• Vinay Saunders, Jennifer M. Dewing, Tilman Sanchez-Elsner, David I. Wilson Expression and localisation of thymosin beta-4 in the developing human early fetal heart PLOS ONE 2018-11-09 [PMID: 30412598] (Immunohistochemistry, Human)
• J Bourland, J Fradette, FA Auger Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development Sci Rep, 2018-09-04;8(1):13191. 2018-09-04 [PMID: 30181613] (IHC, Human)
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Review for CD31/PECAM-1 Antibody (AF806) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ivan Osinnii IHC-Fr Human 08/21/2023

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: colon,Human Colon
• Species: Human
• Lot: DGX0523031

Comments

• Comments: Immunofluorescence on freshly-frozen PFA-fixed cryosections

Bioinformatics

• Gene Symbol: PECAM1