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CD3 epsilon Antibody (OKT-3 (muromonab))

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Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (OKT-3 (muromonab)) [NBP2-81031] - Immunofluorescence staining of fixed Molt4 cells with CD3 epsilon Antibody (OKT-3 (muromonab)) [NBP2-81031]. Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of OKT-3 [NBP2-81031] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81031, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: CD3 epsilon Antibody (OKT-3 (muromonab)) [NBP2-81031] - Immunofluorescence staining of fixed Molt4 cells with CD3 epsilon Antibody (OKT-3 (muromonab)) [NBP2-81031]. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
OKT-3 (muromonab)
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-81031AF350
Alexa Fluor 405
NBP2-81031AF405
Alexa Fluor 488
NBP2-81031AF488
Alexa Fluor 532
NBP2-81031AF532
Alexa Fluor 594
NBP2-81031AF594
Alexa Fluor 647
NBP2-81031AF647
Alexa Fluor 700
NBP2-81031AF700
Alexa Fluor 750
NBP2-81031AF750
Allophycocyanin
NBP2-81031APC
Biotin
NBP2-81031B
DyLight 350
NBP2-81031UV
DyLight 405
NBP2-81031V
DyLight 488
NBP2-81031G
DyLight 550
NBP2-81031R
DyLight 594
NBP2-81031DL594
DyLight 650
NBP2-81031C
DyLight 680
NBP2-81031FR
DyLight 755
NBP2-81031IR
FITC
NBP2-81031F
Janelia Fluor 549
NBP2-81031JF549
Janelia Fluor 646
NBP2-81031JF646
PE
NBP2-81031PE
PerCP
NBP2-81031PCP
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CD3 epsilon Antibody (OKT-3 (muromonab)) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Human peripheral E+ cells.
Specificity
Epitope on the epsilon-subunit within the human CD3 complex.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
CD3E
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD3 epsilon Antibody (OKT-3 (muromonab))

  • CD3 epsilon
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: CD3 delta Antibody (2R1O10) [NBP3-16881] - Immunofluorescence analysis of mouse spleen using CD3 delta Rabbit mAb (NBP3-16881) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: CD3 delta Antibody (2R1O10) [NBP3-16881] - Western blot analysis of extracts of Jurkat cells, using CD3 delta Rabbit mAb (NBP3-16881) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
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Bioinformatics

Gene Symbol CD3E