CD1a Antibody (O10+C1A/711) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10+C1A/711).

Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) - Azide and BSA Free [NBP2-34698] - Paraffin-embedded human tonsil epithelium. Image from verified customer review.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, CyTOF-ready
• Clone: O10+C1A/711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

CD1a Antibody (O10+C1A/711) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34314).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human thymus cells (O10); Recombinant human CD1a protein (C1A/711)
• Localization: Cell surface
• Marker: Mature Langerhans Cells Marker
• Isotype: IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD1A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD1a Antibody (O10+C1A/711) - Azide and BSA Free

• CD1
• CD1a antigen
• CD1A antigen, a polypeptide
• CD1a molecule
• CD1a
• cluster of differentiation 1 A
• cortical thymocyte antigen CD1A
• differentiation antigen CD1-alpha-3
• epidermal dendritic cell marker CD1a
• FCB6
• hTa1 thymocyte antigen
• HTA1
• R4
• T6
• T-cell surface antigen T6/Leu-6
• T-cell surface glycoprotein CD1a

Background

At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD1a Antibody (NBP2-34698) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Immunofluorescence - paraffin Human 09/23/2018

Summary

• Application: Other
• Sample Tested: human tonsil
• Species: Human
• Lot: I don't have it any more

Comments

• Comments: pH9 antigen retrieval

Bioinformatics

• Gene Symbol: CD1A