CD1a Antibody (O10+C1A/711)

Images

 
Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) [NBP2-34314] - Paraffin-embedded human tonsil epithelium. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) [NBP2-34314] - Formalin-fixed, paraffin-embedded human skin stained with CD1a MAb (O10+C1A/711).
Immunohistochemistry-Paraffin: CD1a Antibody (O10+C1A/711) [NBP2-34314] - Analysis of CD1a on human tonsil tissue. 1ug/mL primary antibody. Heat induced epitope retrieval in a basic buffer. Image from a verified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
O10+C1A/711
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD1a Antibody (O10+C1A/711) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34698)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Human thymus cells (O10); Recombinant human CD1a protein (C1A/711)
Localization
Cell surface
Marker
Mature Langerhans Cells Marker
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-34314 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD1a Antibody (O10+C1A/711)

  • CD1
  • CD1a antigen
  • CD1A antigen, a polypeptide
  • CD1a molecule
  • CD1a
  • cluster of differentiation 1 A
  • cortical thymocyte antigen CD1A
  • differentiation antigen CD1-alpha-3
  • epidermal dendritic cell marker CD1a
  • FCB6
  • hTa1 thymocyte antigen
  • HTA1
  • R4
  • T6
  • T-cell surface antigen T6/Leu-6
  • T-cell surface glycoprotein CD1a

Background

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD1a Antibody (NBP2-34314) (0)

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Review for CD1a Antibody (NBP2-34314) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34314:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD1a NBP2-34314
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 09/14/2023
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lot909-3P170425

Comments

Comments1ug/mL primary antibody
heat induced epitope retrieval in a basic buffer
BOND refine kit

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for CD1a Antibody (NBP2-34314) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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2
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1
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Verified Customer
09/14/2023
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CD1A
Uniprot