CD163 Antibody (RM3/1) Summary
Human monocytes (epitope recognized SRCR domain 9)
Protein A or G purified
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Packaging, Storage & Formulations
Store at 4C. Do not freeze.
0.2 um filtered solution in PBS and 0.1% BSA
0.02% Sodium Azide
Protein A or G purified
- Western Blot 1:10
- Flow Cytometry 1:10
- Immunocytochemistry/Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10
- Immunoprecipitation 1:10-1:500
This antibody works in Flow Cytometry, Functional, Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot. The typical starting working dilution is 1:10. CD163 is sensitive to ionic detergents and to chloroform/methanol treatment.
Positive control: CHO-cells transfected with CD163 Negative control: Muscle cells
Alternate Names for CD163 Antibody (RM3/1)
- CD163 antigenscavenger receptor cysteine-rich type 1 protein M130
- CD163 molecule
- Hemoglobin scavenger receptor
- M130macrophage-associated antigen
The monoclonal antibody RM3/1 recognizes CD163, a 130 kDa type I membrane glycoprotein, which is expressed exclusively in the monocyte/macrophage system. CD163 is a member of the cysteine-rich scavenger receptor superfamily. CD163 is an acute phase regulated receptor for the hemoglobin-haptoglobin complex. Another important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels are found on tissue macrophages whereas bone marrow-derived cells are CD163 negative. CD163 positive cells include skin histiocytes, gut, Kupffer cells, and macrophages in spleen, thymus placenta and inflamed or tumorous tissues. The protein expression is markedly induced by glucocorticoids, IL-6 and IL-10 while down-regulated by cyclosporin A and by phorbol esters.
CD163 can be cleaved to release to soluble form (sCD163). PMA can induce the shedding of sCD163. Intravenous lipopolysaccharide (LPS) produces a rapid rise of sCD163 in plasma of patient as it induces metalloproteinase-mediated shedding from monocytes surface. sCD163 in plasma is a parameter in diseases affecting macrophage function and monocyte/macrophage load in the body. The concentration of sCD163 is probably reflecting the number of macrophages of the 'alternative macrophage activation' phenotype with a high CD163 expression playing a major role in dampening the inflammatory response and scavenging components of damaged cells. As such sCD163 can be important as a disease marker in inflammatory conditions e.g. infection, autoimmune disease, transplantation, atherosclerosis and cancer.
The monoclonal antibody RM3/1 can be used for macrophage phenotyping..The RM3/1 antigen expression is restricted to human monocytes and macrophages that evolve in the late phase of inflammation, predominantly macrophages of healing tissue. For macrophages in the synovialis of patients with rheumatoid arthritis; in alveolar macrophages and in Kupffer cells a double staining can be observed with the monoclonal antibody 25F9 (HM2158) recognizing mature macrophages, which is not the case in other tissues.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Publications for CD163 Antibody (NBP1-60158)(5)
Showing Publications 1 - 5
|Publications using NBP1-60158
|Komohara Y, Hirahara J, Horikawa T, Kawamura K, Kiyota E, Sakashita N, Araki N, Takeya M. AM-3K, an anti-macrophage antibody, recognizes CD163, a molecule associated with an anti-inflammatory macrophage phenotype. J Histochem Cytochem;54(7):763-71. 2006 Jul. [PMID:16517975]
|Frings W, Dreier J, Sorg C. Only the soluble form of the scavenger receptor CD163 acts inhibitory on phorbol ester-activated T-lymphocytes, whereas membrane-bound protein has no effect. FEBS Lett. 526(1-3):93-6. 2002 Aug 28. [PMID:12208511]
|Droste A, Sorg C, Hogger P. Shedding of CD163, a novel regulatory mechanism for a member of the scavenger receptor cysteine-rich family. Biochem Biophys Res Commun;256(1):110-3. 1999 Mar 5. [PMID:10066432]
|Hogger P, Dreier J, Droste A, Buck F, Sorg C. Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163). J Immunol;161(4):1883-90. 1998 Aug 15. [PMID:9712057]
|Zwadlo G, Voegeli R, Osthoff KS, Sorg C. A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol;55(6):295-304. 1987 [PMID:3450546]
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