CD163 Antibody (ED2) - BSA Free

Immunocytochemistry/Immunofluorescence: CD163 Antibody (ED2) [NBP2-39099] - Staining of rat lymph node cryosection. Image A in Red (NBP2-39099), and Image B in green using Mouse anti-Rat CD8. Image C is the merged image with nuclei counter-stained blue using DAPI. High power.

Immunohistochemistry-Paraffin: CD163 Antibody (ED2) - BSA Free [NBP2-39099] - Alcohol-fixed paraffin embedded spleen tissue from healthy rat spleen. Primary antibody dilution: 1:100. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: CD163 Antibody (ED2) [NBP2-39099] - Staining of rat lymph node cryosection. Image A in Red (NBP2-39099), and Image B in green using Mouse anti-Rat CD8. Image C is the merged image with nuclei counter-stained blue using DAPI. Low power.

Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Staining of acetone fixed, cryostat sectioned rat spleen.

Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Immunoperoxidase staining of rat lymph node cryosection (NBP2-39099) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power.

Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Immunoperoxidase staining of rat lymph node cryosection (NBP2-39099) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power.

• Reactivity: Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: ED2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD163 Antibody (ED2) - BSA Free Summary

• Immunogen: Rat spleen cell homogenate
• Specificity: This antibody recognizes the rat ED2 cell surface glycoprotein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD163
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Frozen 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
  This antibody may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde). See Whiteland et al.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for CD163 Antibody (ED2) - BSA Free

• CD_antigen: CD163
• CD163 molecule
• CD163
• GHI/61
• HbSR
• Hemoglobin scavenger receptor
• M130
• macrophage-associated antigen
• MM130
• RM3/1
• SCARI1
• scavenger receptor cysteine-rich type 1 protein M130
• sCD163
• Soluble CD163

Background

CD163 (Cluster of Differentiation 163), also known by several other names including M130, p155, RM3/1, Ki-M8, Ber-MAC3, SM4, and GHI/61, is a type I transmembrane glycoprotein that is a member of the scavenger receptor cysteine-rich (SRCR) super family class B (1-3). CD163 is expressed specifically on the monocyte/macrophage lineage and has a theoretical molecular weight of 130-160 kDa in reducing conditions and 110 kDa in non-reducing conditions (2 - 6). CD163 is synthesized as 1076 amino acid (aa) protein consisting of a large extracellular domain (ECD) with nine SRCR domains, proline-serine-threonine rich (PST) linker domain, a transmembrane segment, and a cytoplasmic tail which has varying lengths depending on the isoform due to alternative splicing, with the short 49 aa form being the most common (1-3, 6).

One of the primary functions of CD163 is uptake of haptoglobin-hemoglobin (Hp-Hb) complexes from the liver, spleen, and bone marrow, ultimately triggering an anti-inflammatory response (3, 5, 7). CD163 also functions as an erythroblast adhesion receptor and promotes cell maturation and survival (3, 5, 7). Furthermore, CD163 functions in immune sensing of bacteria and as a receptor for tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) (3, 5, 7). As mentioned above, CD163 is expressed on cells in the monocyte/macrophage lineage and, in general, anti-inflammatory signals including glucocorticoids, interleukin (IL)-6, and IL-10 stimulate CD163 synthesis and expression while, conversely, pro-inflammatory signals such as interferon-gamma (INF-gamma), TNF-alpha, and lipopolysaccharide (LPS) downregulate CD163 (3, 5). In addition to membrane-bound form of CD163, the protein can be cleaved by metalloproteinases (MMP) and induced by LPS or phorbol myristate acetate (PMA) to release a soluble form (sCD163) into the plasma (7). Increased levels of sCD163 in the plasma and an increased number of CD163-expressing macrophages at the site of inflammation are associated with a variety of pathologies (3, 5-7). CD163/sCD163 is often increased and a suitable clinical marker for inflammatory diseases including rheumatoid arthritis (RA), Gaucher disease, chronic kidney disease, diabetes, and Crohn's disease (3, 5-7).

Alternative names for CD163 includes GHI/61, HbSR, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, RM3/1, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, sCD163, and soluble CD163.

References

1. Law, S. K., Micklem, K. J., Shaw, J. M., Zhang, X. P., Dong, Y., Willis, A. C., & Mason, D. Y. (1993). A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. European journal of immunology. https://doi.org/10.1002/eji.1830230940

2. Onofre, G., Kolackova, M., Jankovicova, K., & Krejsek, J. (2009). Scavenger receptor CD163 and its biological functions. Acta medica (Hradec Kralove).

3. Van Gorp, H., Delputte, P. L., & Nauwynck, H. J. (2010). Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy. Molecular immunology. https://doi.org/10.1016/j.molimm.2010.02.008

4. Sulahian, T. H., Hogger, P., Wahner, A. E., Wardwell, K., Goulding, N. J., Sorg, C., Droste, A., Stehling, M., Wallace, P. K., Morganelli, P. M., & Guyre, P. M. (2000). Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. https://doi.org/10.1006/cyto.2000.0720

5. Etzerodt, A., & Moestrup, S. K. (2013). CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxidants & redox signaling. https://doi.org/10.1089/ars.2012.4834

6. Skytthe, M. K., Graversen, J. H., & Moestrup, S. K. (2020). Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases. International journal of molecular sciences, 21(15), 5497. https://doi.org/10.3390/ijms21155497

7. Moller H. J. (2012). Soluble CD163. Scandinavian journal of clinical and laboratory investigation. https://doi.org/10.3109/00365513.2011.626868

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD163 Antibody (NBP2-39099)(7)

Publications using NBP2-39099

• Al-Qazazi R, Lima PDA, Prisco SZ Et al. Macrophage-NLRP3 Activation Promotes Right Ventricle Failure in Pulmonary Arterial Hypertension Am J Respir Crit Care Med 2022-06-14 [PMID: 35699679] (FLOW, Rat)
  
  Details:
  Citation using the Alexa Fluor 700 version of this antibody.
• Park GY, Tarafder S, Eyen SL et al. Oxo-M and 4-PPBP Delivery via Multi-Domain Peptide Hydrogel Toward Tendon Regeneration Frontiers in bioengineering and biotechnology 2022-01-27 [PMID: 35155388] (ICC/IF, Rat)
• Yang P, Zhang X, Lin Z et al. Adoptive transfer of polarized M2c macrophages ameliorates acute rejection in rat liver transplantation Am J Transl Res 2020-06-15 [PMID: 32655794]
• Orsi M CHARACTERIZATION OF A NEW INTERMEDIATE MACROPHAGE SUBPOPULATION: SDC-1-POSITIVE SPM-LIKE MACROPHAGES POSSESS IMMUNOSUPPRESSIVE FUNCTIONS IN EARLY MESOTHELIOMAGENIC RESPONSES TO CARBON NANOTUBES Thesis 2021-01-01
• Gunasekaran M, Mishra R, Saha P et al. Comparative efficacy and mechanism of action of cardiac progenitor cells after cardiac injury iScience 2022-06-01 [PMID: 35847554] (FLOW, Human)
• Isali I, Mcclellan P, Shankar E Et Al. Genipin guides and sustains the polarization of macrophages to the pro-regeneRative M2 subtype via activation of the pSTAT6-PPAR-gamma pathway Acta biomaterialia 2021-07-02 [PMID: 34224892] (FLOW)
• Orsi M, Palmai-Pallag M, Yakoub Y et al. Monocytic Ontogeny of Regenerated Macrophages Characterizes the Mesotheliomagenic Responses to Carbon Nanotubes Frontiers in Immunology 2021-06-14 [PMID: 34194430] (Rat)

Review for CD163 Antibody (NBP2-39099) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Federica Gnudi IHC-P Rat 11/30/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Spleen tissue
• Species: Rat
• Lot: 156103

Comments

• Comments: Dilution: 1:100

Bioinformatics

• Gene Symbol: CD163
• Uniprot:
  • Q86VB7()