CD163 Antibody (10D6)

Images

 
Immunohistochemistry-Paraffin: CD163 Antibody (10D6) [NB110-59935] - Human lymph node stained with CD163 antibody showing cytoplasmic staining in non-germinal center cells. Image from verified customer review.
Immunohistochemistry-Paraffin: CD163 Antibody (10D6) [NB110-59935] - Human tonsil.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
10D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD163 Antibody (10D6) Summary

Immunogen
Human CD163 antigen.
Localization
Membrane
Marker
Histiocytic Marker
Specificity
This CD163 molecule is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 protein is restricted in its expression to the monocytic/macrophage lineage. It is reported to be present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells, and Langerhans cells. Multinucleated cells within inflammatory lesions are reported not to express CD163 protein. The protein is upregulated by glucocorticoids and is downregulated by immunosuppressant cyclosporin A and phorbol esters.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD163
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:50
Positive Control
CD163 Lysate (NBL1-08909)
Reviewed Applications
Read 1 Review rated 4
using
NB110-59935 in the following applications:

Publications
Read Publications using
NB110-59935 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD163 Antibody (10D6)

  • CD163 antigenscavenger receptor cysteine-rich type 1 protein M130
  • CD163 molecule
  • CD163
  • GHI/61
  • HbSR
  • Hemoglobin scavenger receptor
  • M130
  • M130macrophage-associated antigen
  • MM130
  • RM3/1

Background

CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD163 Antibody (NB110-59935)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(3)
IHC
(5)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB110-59935 Applications Species
Lundgren S, Karnevi E, Elebro J et al. The clinical importance of tumour-infiltrating macrophages and dendritic cells in periampullary adenocarcinoma differs by morphological subtype J Transl Med 2017 Jul 03 [PMID: 28673320] (IHC, Human) IHC Human
Burgess M, Mapp S, Mazzieri R, Cheung C Increased FcRIIB dominance contributes to the emergence of resistance to therapeutic antibodies in chronic lymphocytic leukaemia patients. Oncogene Jul 8 1905 [PMID: 27748757] (ICC/IF, Human) ICC/IF Human
Herbst Rs, Soria Jc, Kowanetz M et al. Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients. Nature. 2014 Nov 27 [PMID: 25428504] (IHC) IHC
Gadd VL, Melino M, Roy S et al. Portal, but not lobular, macrophages express matrix metalloproteinase-9: association with the ductular reaction and fibrosis in chronic hepatitis C Liver Int 2012 Nov 12 [PMID: 23240894] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Melino M, Gadd VL, Walker GV, Skoien R, Barrie HD, Jothimani D, Horsfall L, Jones A, Sweet MJ, Thomas GP, Clouston AD, Jonsson JR, Powell EE. Macrophage secretory products induce an inflammatory phenotype in hepatocytes. World J Gastroenterol;18(15):1732-44. 2012 Apr 21. [PMID: 22553397] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Fealey ME, Edwards WD, Miller DV, Maleszewski JJ. Unicommissural aortic valves: gross, histological, and immunohistochemical analysis of 52 cases (1978-2008). Cardiovasc Pathol. 2011 Dec 27. [PMID: 22206734] (IHC, Human) IHC Human

Review for CD163 Antibody (NB110-59935) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB110-59935:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD163 NB110-59935
Enlarge
reviewed by:
Anonymous
IHC-P Human 10/06/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLymph node
SpeciesHuman
LotE097

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Recent Reviews

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Anonymous
10/06/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CD163
Entrez
Uniprot