CD11c Antibody (BU15) - BSA Free

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Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) Pacific BlueTM.
Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP (monocyte gate).
Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) APC.
Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP-CyTM5.5.
Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PE.
Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PE-CyTM7. .
Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) PerCP.
Flow Cytometry: CD11c Antibody (BU15) [NBP1-45018] - Staining of human peripheral blood cells with anti-CD11c (BU15) FITC.
Separation of human monocytes (red-filled) from CD11c negative lymphocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood stained using anti-human CD11c (BU15) purified antibody ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, IHC, IP, CyTOF-ready
Clone
BU15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

CD11c Antibody (BU15) - BSA Free Summary

Additional Information
Clone BU15 was used by HLDA to establish CD designation.
Immunogen
Dendritic cells of synovial fluid
Marker
Dendritic Cell Marker
Specificity
The antibody BU15 reacts with CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages. HLDA III; WS Code M 256 HLDA V; WS Code AS S143 HLDA VI; WS Code AS Ref.6
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGAX
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
Application Notes
This antibody is Cytof ready.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-45018 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD11c Antibody (BU15) - BSA Free

  • 95 alpha chain
  • 95
  • 95, alpha subunit
  • CD11 antigen-like family member C
  • CD11c antigen
  • CD11c
  • CD11Cleu M5, alpha subunit
  • Integrin alpha X
  • integrin alpha-X
  • integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
  • integrin, alpha X (complement component 3 receptor 4 subunit)
  • ITGAX
  • Leu M5
  • Leukocyte adhesion glycoprotein p150
  • Leukocyte adhesion receptor p150
  • leukocyte surface antigen p150
  • myeloid membrane antigen, alpha subunit
  • p150 95 integrin alpha chain
  • p150,95 alpha
  • SLEB6

Background

CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11c Antibody (NBP1-45018)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Product General Protocols

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FAQs for CD11c Antibody (NBP1-45018). (Showing 1 - 1 of 1 FAQ).

  1. Is the epitope for antibody clone BU15 intracellular or extracellular?
    • The epitope for this antibody is extracellular.

Secondary Antibodies

 

Isotype Controls

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Blogs on CD11c.


  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

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Bioinformatics

Gene Symbol ITGAX