CD117/c-kit Antibody (R21-V)

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (R21-V) [NBP1-79010] - Membranous CD117 positivity in the classical seminoma. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti-CD117 monospecific clonal antibody.

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (R21-V) [NBP1-79010] - Membranous CD117 positivity in the classical seminoma. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - CD117.

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (R21-V) [NBP1-79010] - Membranous CD117 positivity in the classical seminoma. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti-CD117 monospecific clonal antibody.

Product Discontinued

CD117/c-kit Antibody (R21-V) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Peptide derived from C-terminal sequence of human CD117/c-kit. Antibody recognizes the epitope between Ile957 - His973.
• Epitope: Ile957 - His973
• Marker: Hematopoietic Stem Cell Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KIT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100-1:200

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for CD117/c-kit Antibody (R21-V)

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIT
• Uniprot:
  • P10721(Human)