CD117/c-kit Antibody (C117/370 + KIT/982 + KIT/983) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370 + KIT/982 + KIT/983) - Azide and BSA Free [NBP2-47845] - Human skin angiosarcoma (FFPE tissue) stained with c-kit. pH 9 HIER antigen retrieval. Image from verified customer review.

Product Discontinued

CD117/c-kit Antibody (C117/370 + KIT/982 + KIT/983) - Azide and BSA Free Summary

• Immunogen: Recombinant human CD117 proteins
• Localization: Cell surface and cytoplasmic
• Marker: Marker for Gastrointestinal Stromal Tumors
• Specificity: This MAb recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
• Isotype: IgG1 Kappa/IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 4q12 Molecular weight of antigen: ~145kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD117/c-kit Antibody (C117/370 + KIT/982 + KIT/983) - Azide and BSA Free

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD117/c-kit Antibody (NBP2-47845) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Immunofluorescence - paraffin Human 01/20/2019

Summary

• Application: Other
• Sample Tested: human angiosarcoma
• Species: Human
• Lot: I don't have it any more

Comments

• Comments: pH 9 antigen retrieval

Bioinformatics

• Gene Symbol: KIT