CD117/c-kit Antibody (ACK2) [PE]

Product Discontinued

CD117/c-kit Antibody (ACK2) [PE] Summary

• Immunogen: Mouse CD117
• Marker: Hematopoietic Stem Cell Marker
• Specificity: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: KIT
• Purity: Protein A or G purified
• Endotoxin Note: Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247). Guinea Pig reactivity reported in scientific literature (PMID: 21654812)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD117/c-kit Antibody (ACK2) [PE]

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (NBP1-43821)(2)

Publications using NBP1-43821

• Meyer S, Maufort JP, Nie J et al. Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents PLoS One 2013-08-23 [PMID: 23967247] (FLOW, Human)
• Fu W, Song B, Li W et al. Ultrastructural features and possible functional role of kit-positive interstitial cells in the guinea pig corpus cavernosum Int J Impot Res 2011-06-10 [PMID: 21654812] (FLOW, Guinea Pig)

Bioinformatics

• Gene Symbol: KIT