CD117/c-kit Antibody (8D7)

Product Discontinued

CD117/c-kit Antibody (8D7) Summary

• Immunogen: Purified recombinant fragment of C-kit expressed in E. Coli.
• Marker: Hematopoietic Stem Cell Marker
• Specificity: CD117 (5C8D4)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for CD117/c-kit Antibody (8D7)

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

C-kit(145kDa) functions as a tyrosine kinase receptor which becomes activated upon binding of its ligand SCF (stem-cell factor), the C-kit gene encodes the human homolog of the proto-oncogene c-kit. which was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (NB100-1766)(3)

Publications using NB100-1766

• Motta ACF, Zhan Q, Larson A et al. Immunohistopathological characterization and the impact of topical immunomodulatory therapy in oral chronic GVHD: a pilot study Oral Dis [PMID: 29197137] (IF/IHC, Human)
• Umbreit C, Erben P, Faber A et al. Lapatinib-induced mesenchymal-epithelial transition in squamous cell carcinoma cells correlates with unexpected alteration of beta-catenin expression. Oncol Lett 2016-04-01 [PMID: 27073542] (IF/IHC)
• Schultz JD, Rotunno S, Erben P et al. Down-regulation of MMP-2 expression due to inhibition of receptor tyrosine kinases by imatinib and carboplatin in HNSCC. Oncol Rep 2011-04-01 [PMID: 21249321]

Bioinformatics

• Gene Symbol: KIT
• Entrez:
  • 3815(Human)
• Uniprot:
  • P10721()