CD117/c-kit Antibody (2B8) - Azide and BSA Free

Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113). Image from the standard format of this antibody.

Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC. Image from the standard format of this antibody.

Flow Cytometry: CD117/c-kit Antibody (2B8) - Azide and BSA Free [NBP2-80624] - Identification of CD117/c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and rabbit anti-CD117/c-kit antibody 2B8. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0071798) licensed under a CC-BY license. Image from the standard format of this antibody.

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 2B8
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD117/c-kit Antibody (2B8) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat CD117/c-kit Antibody (2B8) - Azide and BSA Free (NB100-77477) is a monoclonal antibody validated for use in IHC, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mouse bone marrow mast cells
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: KIT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.6 ug/10^6 cells in 100 uL
  • Immunocytochemistry/ Immunofluorescence 0.25 ug/10^6 cells in 100 uL. Use reported in scientific literature (PMID 28097227)
  • Immunohistochemistry 1:20 - 1:1000
  • Immunohistochemistry-Frozen 1:20 - 1:1000
  • Immunoprecipitation 1:20 - 1:1000
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD117/c-kit Antibody (2B8) - Azide and BSA Free

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIT