CD117/c-kit Antibody (017)

Western Blot: CD117/c-kit Antibody (017) [NBP2-90624] - Anti-CD117/c-kit rabbit monoclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 110 kDa. Observed band size: 110 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: CD117/c-kit Antibody (017) [NBP2-90624] - Mouse CD117/c-kit was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 1 ul anti-Mouse CD117/c-kit rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse CD117/c-kit rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 110 kDa. Observed band size: 110 kDa

Product Discontinued

CD117/c-kit Antibody (017) Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD117/c-kit (NP_001116205.1; Met1-Thr523).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KIT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 0.2-1 ul/mg of lysate
  • Tissue Culture Substratum
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CD117/c-kit Antibody (017)

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIT