CD11 Antibody (24) - BSA Free

• Reactivity: Hu
• Applications: WB, Flow, Func, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 24
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD11 Antibody (24) - BSA Free Summary

• Description: Novus Biologicals Mouse CD11 Antibody (24) - BSA Free (NB100-2623) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-CD11 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fibronectin-purified human monocytes.
• Epitope: Activation epitope on integrin beta 2 (CD18) subunit
• Localization: Cell Membrane
• Marker: Microglia Marker, Macrophage Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Radioimmunoassay 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000
• Application Notes: Clone 24 may be used as a reporter of the activation state of the CD11/CD18 integrins and can also be induced following exposure with Mg2+ or Mn2+. CD18: 95 kDa, CD11: 180kDa. This antibody is CyTOF ready. Positive control(s): Tonsil.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD11 Antibody (24) - BSA Free

• antigen CD11b (p170)
• CD11 antigen-like family member B
• CD11b antigen
• CD11B integrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide)
• CD11b
• Cell surface glycoprotein MAC-1 subunit alpha
• CR-3 alpha chain
• CR3AMGC117044
• integrin alpha-M
• integrin, alpha M (complement component 3 receptor 3 subunit)
• Leukocyte adhesion receptor MO1
• MAC-1
• MAC1A
• macrophage antigen alpha polypeptide
• MO1A
• neutrophil adherence receptor alpha-M subunit
• Neutrophil adherence receptor
• SLEB6

Background

The CD11/18 complex of glycoproteins are dimers comprised of a common beta chain and individual alpha chains (CD11a/18 or CD11b/18 and CD11c/18). CD11a/18 is a receptor for C3bi, a complement component, CD11b/18 is Lymphocyte Function Activator, and CDD11c/18 is a lectin-like glycoprotein thought to be important for mediation of cells to other surfaces.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD11 Antibody (NB100-2623)(2)

Publications using NB100-2623

• Sharfe N, Karanxha A, Dadi H et al. Dual loss of p110d PI3-kinase and SKAP (KNSTRN) expression leads to combined immunodeficiency and multisystem syndromic features J. Allergy Clin. Immunol. 2017-11-24 [PMID: 29180244] (Human)
• Donahue RE, Tuschong L, Bauer TR Jr et al. Leukocyte integrin activation mediates transient neutropenia after G-CSF administration Blood 2011-10-13 [PMID: 21844566] (FLOW, Human)

Bioinformatics

• Gene Symbol: ITGAM
• Entrez:
  • 3684(Human)
• Uniprot:
  • P11215(Rat)