CCR9 Antibody (882416)

HEK293 human embryonic kidney cell line transfected with (A) rat CCR9 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CCR9 Monoclonal Antibody (Catalog # MAB8287) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

• Reactivity: Rt
• Applications: Flow, CyTOF-ready
• Clone: 882416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR9 Antibody (882416) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with rat CCR9
• Specificity: Detects rat CCR9 in direct ELISAs. Stains rat CCR9 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR9 Antibody (882416)

• C-C chemokine receptor type 9
• C-C CKR-9
• CC-CKR-9
• CCR9
• CCR-9
• CD199
• CDw199 antigen
• CDw199
• chemokine (C-C motif) receptor 9
• G protein-coupled receptor 28
• GPR28
• GPR-9-6G-protein coupled receptor 28

Background

CCR9 is an approximately 42 kDa (predicted) seven transmembrane domain receptor for the chemokine CCL25/TECK. It is expressed on CD8⁺ T cells, monocytes, dendritic cells, and IgA⁺ plasma cells. It plays a role in oral and gut tolerance, the development of g/d T cells, and the thymic recruitment of hematopoietic progenitor cells. CCR9⁺ cells limit inflammatory cell infiltration in rheumatoid arthritis, hepatitis, colitis, and inflammatory bowel disease, but exacerbate it in atherosclerosis. In the brain, CCR9 is expressed on CA1 region pyramidal neurons in the hippocampus and is upregulated on microglia during T. gondii infection. CCR9 is also upregulated in a variety of cancers where it promotes tumor cell metastasis. Rat CCR9 shares 86% and 98% amino acid sequence identity with human and mouse CCR9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCR9