CCR10/GPR2 Antibody (314305) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Rat Anti-Human CCR10 Monoclonal Antibody (Catalog # MAB3478) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells.Expression of EBNA-2 and LMP-1 in IL-21 treated cells (A, B). (A) Simultaneous immunofluorescence staining of EBNA-2 (Green) and LMP-1 (Red); magnification (×100), scale bar 25 µm. Note the downregulation of EBNA-2 and upregultion of LMP-1 after IL-21 treatment. (B) Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in the control lanes and 5×105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. (C) Activity of the W and C promoters that regulate EBNA-2 expression and LMP-1 mRNA expression by Q-PCR. Note the difference in EBNA-2 regulation; the MEC2 cell uses both Wp and Cp while in MEC1 only Wp is active. (D) Expression of EBNA-2 and LMP-1 in cells exposed to CD40L. Simultaneous immunofluorescence staining; for details see (A). Note: EBNA-2 and LMP-1 are downregulated by CD40L in both lines. (E) CD40L induced modulation of surface marker by FACS analysis. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by R&D Systems.

Comparison of the MEC1 and MEC2 cells.(A) Expression of EBV encoded proteins EBNA-2 and LMP-1 by immunofluorescence; magnification (×100), scale bar 25 µm. Note: the MEC2 cells are larger. (B) Expression of EBNA-2 and LMP-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5×105 cells were loaded in control lanes and 5×105 were loaded in MEC1 and MEC2 lanes. Note MEC2 expresses higher amount of EBNA-2. (C) Expression of Bright and BARF1 by Q-PCR. (D) FACS analysis of surface markers that are differently expressed in the 2 lines. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 314305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR10/GPR2 Antibody (314305) [Unconjugated] Summary

• Immunogen: Y3 rat myeloid cell line transfected with human CCR10
  Gln8-Asn362
  Accession # P46092
• Specificity: Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR10/GPR2 Antibody (314305) [Unconjugated]

• CC chemokine receptor 10
• C-C chemokine receptor type 10
• C-C CKR-10
• CC-CKR-10
• CCR10
• CCR-10
• chemokine (C-C motif) receptor 10
• G protein-coupled receptor 2
• GPR2
• GPR2G-protein coupled receptor 2

Background

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that function as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR10/GPR2 Antibody (MAB3478)(6)

Publications using MAB3478

• Ishan Roy, Kathleen A. Boyle, Emily P. Vonderhaar, Noah P. Zimmerman, Egal Gorse, A. Craig Mackinnon et al. Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma Laboratory Investigation 3/1/2017 [PMID: 28092365]
• McCully M, Collins P, Hughes T, Thomas C, Billen J, O'Donnell V, Moser B Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells. J Immunol, 2015-05-22;195(1):96-104. 2015-05-22 [PMID: 26002980] (Flow Cytometry, Human)
• Davey M, Morgan M, Liuzzi A, Tyler C, Khan M, Szakmany T, Hall J, Moser B, Eberl M Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells. J Immunol, 2014-08-27;193(7):3704-3716. 2014-08-27 [PMID: 25165152] (Flow Cytometry, Human)
• Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia. PLoS ONE, 2014-08-27;9(8):e106008. 2014-08-27 [PMID: 25162594] (Flow Cytometry, Human)
• Duhen T, Campbell D IL-1beta promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes. J Immunol, 2014-06-02;193(1):120-9. 2014-06-02 [PMID: 24890729] (Flow Cytometry, Human)
• Yamanaka K, Dimitroff CJ, Fuhlbrigge RC, Kakeda M, Kurokawa I, Mizutani H, Kupper TS Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression. J. Allergy Clin. Immunol., 2007-10-01;121(1):148-157.e3. 2007-10-01 [PMID: 17910894] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR10
• Uniprot:
  • P46092()