CCR10/GPR2 Antibody (314305) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Rat Anti-Human CCR10 PE-conjugated Monoclonal Antibody (Catalog # FAB3478P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

L1.2 mouse pro-B cell line transfected with human CCR10 and eGFP was stained with either (A) Rat Anti-Human CCR10 PE-conjugated Monoclonal Antibody (Catalog # FAB3478P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 314305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

CCR10/GPR2 Antibody (314305) [Phycoerythrin] Summary

• Immunogen: Y3 rat myeloid cell line transfected with human CCR10
  Gln8-Asn362
  Accession # P46092
• Specificity: Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR10/GPR2 Antibody (314305) [Phycoerythrin]

• CC chemokine receptor 10
• C-C chemokine receptor type 10
• C-C CKR-10
• CC-CKR-10
• CCR10
• CCR-10
• chemokine (C-C motif) receptor 10
• G protein-coupled receptor 2
• GPR2
• GPR2G-protein coupled receptor 2

Background

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27 and CCL28. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression is correlated with the metastatic behavior of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR10/GPR2 Antibody (FAB3478P)(16)

Publications using FAB3478P

• OJ Cordero, C Rafael-Vid, R Varela-Cal, C Calviño-Sa, B Malvar-Fer, S García, JE Viñuela, JM Pego-Reigo Distinctive CD26 Expression on CD4 T-Cell Subsets Biomolecules, 2021-10-02;11(10):. 2021-10-02 [PMID: 34680079] (Flow Cytometry, Human)
• W Szczeklik, B Jakie?a, K Wawrzycka-, M Sanak, M Hubalewska, A Padjas, M Surmiak, K Szczeklik, J Sznajd, J Musia? Skewing towards Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis Eur. J. Immunol, 2017-02-27;0(0):. 2017-02-27 [PMID: 28155222] (Flow Cytometry, Human)
• Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human)
• Lee Faulkner Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity Toxicol. Sci, 2016-09-16;154(2):416-429. 2016-09-16 [PMID: 27637899] (Flow Cytometry, Human)
• G Picarda, C Chéneau, JM Humbert, G Bériou, P Pilet, J Martin, F Duteille, P Perrot, F Bellier-Wa, M Heslan, F Haspot, F Guillon, R Josien, FA Halary Functional Langerinhigh-Expressing Langerhans-like Cells Can Arise from CD14highCD16- Human Blood Monocytes in Serum-Free Condition J Immunol, 2016-03-25;0(0):. 2016-03-25 [PMID: 27016604] (Flow Cytometry, Human)
• F. Hessner, C. P. Dlugos, T. Chehab, C. Schaefer, B. Homey, V. Gerke et al. CC chemokine receptor 10 cell surface presentation in melanocytes is regulated by the novel interaction partner S100A10 Scientific Reports 3/4/2016 [PMID: 26941067]
• Engen S, Valen Rukke H, Becattini S, Jarrossay D, Blix I, Petersen F, Sallusto F, Schenck K The oral commensal Streptococcus mitis shows a mixed memory Th cell signature that is similar to and cross-reactive with Streptococcus pneumoniae. PLoS ONE, 2014-08-13;9(8):e104306. 2014-08-13 [PMID: 25119879] (Immunoprecipitation, Human)
• Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer, 2014-02-22;14(0):118. 2014-02-22 [PMID: 24559071] (Flow Cytometry, Human)
• Buckner , Clarisa, Moir , Susan, Kardava , Lela, Ho , Jason, Santich , Brian H, Kim , Leo Jin, Funk , Emily K, Nelson , Amy K, Winckler , Britanny, Chairez , Cheryl L, Theobald-Whiting , Narda L, Anaya-O'Brien , Sandra, Alimchandani , Meghna, Quezado , Martha M, Yao , Michael, Kovacs , Joseph A, Chun , Tae-Wook, Fauci , Anthony, Malech , Harry L, De Ravin , Suk See CXCR4/IgG-expressing plasma cells are associated with human gastrointestinal tissue inflammation. J Allergy Clin Immunol, 2013-12-25;133(6):1676-85.e5. 2013-12-25 [PMID: 24373354] (Flow Cytometry, Human)
• Tang , Xin-Zi, Jo , Juandy, Tan , Anthony, Sandalova , Elena, Chia , Adeline, Tan , Kai Chah, Lee , Kang Hoe, Gehring , Adam J, De Libero , Gennaro, Bertoletti , Antonio IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells. J Immunol, 2013-02-27;190(7):3142-52. 2013-02-27 [PMID: 23447689] (Flow Cytometry, Human)
• Duhen T, Duhen R, Lanzavecchia A Functionally distinct subsets of human FOXP3+ Treg cells that phenotypically mirror effector Th cells. Blood, 2012-03-21;119(19):4430-40. 2012-03-21 [PMID: 22438251] (Flow Cytometry, Human)
• Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis. Clin. Exp. Immunol., 2010-11-19;163(2):215-24. 2010-11-19 [PMID: 21087446] (Flow Cytometry, Human)
• Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab. Blood, 2010-09-09;116(24):5181-90. 2010-09-09 [PMID: 20829370] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Carbone T, Nasorri F, Pennino D CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses. J. Immunol., 2009-12-11;184(2):1102-10. 2009-12-11 [PMID: 20008290] (Flow Cytometry, Human)
• Kroeze KL, Jurgens WJ, Doulabi BZ, van Milligen FJ, Scheper RJ, Gibbs S Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES. J. Invest. Dermatol., 2009-01-01;129(6):1569-81. 2009-01-01 [PMID: 19122644] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR10
• Uniprot:
  • P46092()