CCR10/GPR2 Antibody (314305) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Rat Anti-Human CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB3478A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

L1.2 mouse pro-B cell line transfected with human CCR10 and eGFP was stained with either (A) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A) or (B) Rat Anti-Human CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB3478A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 314305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

CCR10/GPR2 Antibody (314305) [Allophycocyanin] Summary

• Immunogen: Y3 rat myeloid cell line transfected with human CCR10
  Gln8-Asn362
  Accession # P46092
• Specificity: Detect human CCR10. Stains human CCR10-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR10/GPR2 Antibody (314305) [Allophycocyanin]

• CC chemokine receptor 10
• C-C chemokine receptor type 10
• C-C CKR-10
• CC-CKR-10
• CCR10
• CCR-10
• chemokine (C-C motif) receptor 10
• G protein-coupled receptor 2
• GPR2
• GPR2G-protein coupled receptor 2

Background

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27 and CCL28. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression is correlated with the metastatic behavior of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR10/GPR2 Antibody (FAB3478A)(7)

Publications using FAB3478A

• SY Kim-Wang, AG Holt, AM McGowan, ST Danyluk, AP Goode, BC Lau, AP Toth, JR Wittstein, LE DeFrate, JS Yi, AL McNulty Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries Arthritis Research & Therapy, 2021-11-04;23(1):280. 2021-11-04 [PMID: 34736523] (Flow Cytometry, Human)
• Can Liu, Andrew J. Martins, William W. Lau, Nicholas Rachmaninoff, Jinguo Chen, Luisa Imberti et al. Time-resolved systems immunology reveals a late juncture linked to fatal COVID-19 Cell 4/1/2021 [PMID: 33713619]
• T Karnezis, R Farnsworth, NC Harris, SP Williams, C Caesar, DJ Byrne, P Herle, ML Macheda, R Shayan, YF Zhang, S Yazar, SJ Takouridis, C Gerard, S Fox, MG Achen, SA Stacker CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS Cancer Res., 2019-02-01;0(0):. 2019-02-01 [PMID: 30709930] (Flow Cytometry, Human)
• Danilova , E, Skrindo , I, Gran , E, Hales , B J, Smith , W A, Jahnsen , J, Johansen , F E, Jahnsen , F L, Baekkevold , E S A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa. Mucosal Immunol, 2014-06-11;8(1):107-14. 2014-06-11 [PMID: 24917456] (Flow Cytometry, Human)
• Chihara , Norio, Aranami , Toshimas, Oki , Shinji, Matsuoka , Takako, Nakamura , Masakazu, Kishida , Hitaru, Yokoyama , Kazumasa, Kuroiwa , Yoshiyuk, Hattori , Nobutaka, Okamoto , Tomoko, Murata , Miho, Toda , Tatsushi, Miyake , Sachiko, Yamamura , Takashi Plasmablasts as migratory IgG-producing cells in the pathogenesis of neuromyelitis optica. PLoS ONE, 2013-12-10;8(12):e83036. 2013-12-10 [PMID: 24340077] (Flow Cytometry, Human)
• Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab. Blood, 2010-09-09;116(24):5181-90. 2010-09-09 [PMID: 20829370] (Flow Cytometry, Human)
• Hansson M, Hermansson M, Svensson H, Elfvin A, Hansson LE, Johnsson E, Sjoling A, Quiding-Jarbrink M CCL28 is increased in human Helicobacter pylori-induced gastritis and mediates recruitment of gastric immunoglobulin A-secreting cells. Infect. Immun., 2008-04-21;76(7):3304-11. 2008-04-21 [PMID: 18426876] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR10
• Uniprot:
  • P46092()