Monkey CCL5/RANTES ELISA Kit (Colorimetric)

Standard Curve Reference.

Samples were spiked with high concentrations of Monkey CCL5/RANTES, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Pm
• Applications: ELISA
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Monkey CCL5/RANTES ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 3 h 30 min
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CCL5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Monkey CCL5/RANTES ELISA Kit (Colorimetric)

• CCL5
• chemokine (C-C motif) ligand 5
• D17S136Enormally T-expressed, and presumably secreted
• EoCP
• Eosinophil chemotactic cytokine
• RANTES
• SISd
• SIS-delta
• small inducible cytokine A5 (RANTES)
• small inducible cytokine subfamily A (Cys-Cys), member 5
• Small-inducible cytokine A5
• T cell-specific protein P228
• T-cell specific protein p288
• TCP228T-cell-specific protein RANTES

Background

RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5 (1). This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1 (2). CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CCL5/RANTES ELISA Kit (NBP3-49897). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: CCL5