CCL5/RANTES Antibody (53405.111) - Azide and BSA Free

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 53405.111
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CCL5/RANTES Antibody (53405.111) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat CCL5/RANTES Antibody (53405.111) - Azide and BSA Free (NB120-10394) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-CCL5/RANTES Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full length protein (Mouse) expressed in E.coli.
• Localization: Secreted
• Specificity: RANTES (53405.111)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCL5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 8-25 ug/ml
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: Use at a concentration of 0.5 - 2 ug/ml. The detection limit for mouse RANTES is approximately 50.From ELISA, this antibody shows ~50% cross-reactivity with recombinant human RANTES. Use in ICC/IF was reported in scientific literature (PMID: 31760088). Neutralization : Monoclonal Anti-RANTES is measured by its ability to neutralize the biological activity of recombinant mouse RANTES. This antibody will also neutralize the bioactivity of recombinant human RANTES when used at 100x higher IgG concentration.

Packaging, Storage & Formulations

• Storage: Store at -20C as supplied. After reconstitution, store at 2C to 8C for 1 month and at -20 for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: PBS and 5% Trehalose pH 7.4
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for CCL5/RANTES Antibody (53405.111) - Azide and BSA Free

• CCL5
• chemokine (C-C motif) ligand 5
• D17S136Enormally T-expressed, and presumably secreted
• EoCP
• Eosinophil chemotactic cytokine
• RANTES
• SISd
• SIS-delta
• small inducible cytokine A5 (RANTES)
• small inducible cytokine subfamily A (Cys-Cys), member 5
• Small-inducible cytokine A5
• T cell-specific protein P228
• T-cell specific protein p288
• TCP228T-cell-specific protein RANTES

Background

RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL5/RANTES Antibody (NB120-10394)(3)

Publications using NB120-10394

• Liu T, Liu S, Rui X et al. Gasdermin B, an asthma-susceptibility gene, promotes MAVS-TBK1 signalling and airway inflammation. The European respiratory journal 2024-05-02 [PMID: 38514093]
• Liu Q, Li S, Yu L et al. CCL5 Suppresses Klotho Expression via p-STAT3/DNA Methyltransferase1-Mediated Promoter Hypermethylation Frontiers in Physiology 2022-03-01 [PMID: 35299661] (Immunocytochemistry/ Immunofluorescence, Human)
• Upmanyu N, Bulldan A, Failing K, Scheiner-Bobis G DHEAS prevents pro-metastatic and proliferative effects of 17 beta-estradiol on MCF-7 breast cancer cells Biochim Biophys Acta Mol Cell Res 2019-11-21 [PMID: 31760088] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CCL5
• Entrez:
  • 6352(Human)
  • 20304(Mouse)