CCL25/TECK Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CCL25/TECK Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse CCL25/TECK (R&D Systems, Catalog # 481-TK)
  Gln24-Asn144
  Accession # O35903
• Specificity: Detects mouse CCL25/TECK in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human CCL25/TECK is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL25
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CCL25/TECK Antibody (Catalog # MAB4811)
  ELISA Detection: Mouse CCL25/TECK Biotinylated Antibody (Catalog # BAF481)
  Standard: Recombinant Mouse CCL25/TECK (Catalog # 481-TK)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL25/TECK Antibody [Biotin]

• C-C motif chemokine 25
• CCL25
• chemokine (C-C motif) ligand 25
• Chemokine TECK
• Ck beta-15
• Ckb15
• SCYA25TECKvar
• small inducible cytokine subfamily A (Cys-Cys), member 25
• Small-inducible cytokine A25
• TECK
• TECKMGC150327
• thymus expressed chemokine
• Thymus-expressed chemokine

Background

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Human CCL25 cDNA has also been cloned and shown to encode a 151 amino acid protein that exhibits 49% amino acid sequence identity to mouse CCL25. The expression of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered.

1. Vicari, A.P. et al. (1997) Immunity 7:291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL25/TECK Antibody (BAF481)(2)

Publications using BAF481

• Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction. Eur. J. Immunol., 2012-05-01;42(5):1250-60. 2012-05-01 [PMID: 22539297] (ELISA Development, Mouse)
• Onai N, ?< |ƒP4¡6, Kitabatake M, Zhang YY, Ishikawa H, Ishikawa S, Matsushima K Pivotal role of CCL25 (TECK)-CCR9 in the formation of gut cryptopatches and consequent appearance of intestinal intraepithelial T lymphocytes. 6FÙFf‡ÅæÂ$+€absˆÍ”yi‚ 2002-07-01 [PMID: 12096027] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: CCL25
• Uniprot:
  • O35903()