Recombinant Human MAdCAM-1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human MAdCAM-1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 104 cells/well are added to recombinant human MAdCAM-1/Fc Chimera coated plates (2.5 µg/mL with 100 µL/well), approximately 50%-70% will adhere after 1 hour incubation at 37 °C in the presence of 1 mM MnCl2.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human MAdCAM-1 protein
Human MAdCAM-1
Met1-Gln333
Accession # AAY82472
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Val23 & Glu29
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
MADCAM1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
59.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-100 kDa, reducing conditions
Publications
Read Publications using
6056-MC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MAdCAM-1 Fc Chimera Protein, CF

  • hMAdCAM-1
  • MACAM1
  • MAdCAM1
  • MAdCAM-1
  • mucosal addressin cell adhesion molecule 1
  • mucosal addressin cell adhesion molecule-1
  • mucosal vascular addressin cell adhesion molecule 1

Background

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an approximately 60 kDa type 1 transmembrane glycoprotein. It is an endothelial cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily of proteins (1). Human MAdCAM-1 is synthesized as a 382 amino acid (aa) precursor that contains an 18 aa signal sequence, a 299 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD there is one potential site for N-linked glycosylation (2). The ECD comprises two Ig-like domains of 90 aa and 119 aa, respectively, each possessing invariant cysteine residues that stabilize the Ig loop (2). There is also a Ser-Thr-Pro-rich (71%) mucin-like 48 aa domain that is (aa 206 ‑ 317) formed by six tandem repeats of an eight aa sequence having the general consensus DTTSPEP/SP. This mucin domain contains 19 potential sites for O-linked glycosylation (2, 3). A splicing variant in which a single Ala residue is substituted for aa 223 ‑ 334 in isoform 1 produces a second isoform. Human mature MAdCAM-1 shares only 44% aa sequence identity with mature mouse MAdCAM-1. The integrin alpha (4)  beta (7), which is expressed on lymphocytes, functions as the MAdCAM-1 receptor (1). The Ig domains of MAdCAM-1 are critical to alpha (4)  beta (7)  binding, and the mucin domain has activity in L‑Selectin binding. MAdCAM-1 expression is up-regulated by TNF-alpha  and IL‑1 beta. MAdCAM-1 is expressed on the surface of high endothelial venules (HEV) in the gut and in Peyer’s patches, on endothelial cells of the mesenteric lymph nodes, lamina propria of the small and large intestine, and the mammary gland during lactation, and on brain endothelial cells (1). MAdCAM‑1 has also been reported to be expressed in the liver portal region in autoimmune hepatitis (1), and in bone marrow following allogenic (genetically non-identical) hematopoietic stem cell transplantation, where it recruits donor T cells, which may lead to graft versus host disease (3, 4). MAdCAM‑1 functions as a homing receptor, and plays a central role in leukocyte migration into HEVs and Peyer’s patch (5). In addition to its normal role in lymphocyte trafficking to mucosal tissue, MAdCAM‑1 expression is also dramatically increased in chronic inflammatory and disease states (1, 6), including inflammatory bowel disease (Crohn’s disease and ulcerative colitis) (7), sclerosing cholangitis (8), and diabetes (9), and may play an important role in these conditions.
  1. Ando, T. et al. (2007) BMC Physiol. 7:10.
  2. Dando, J. et al. (2002) Acta Crystallogr. D 58:233.
  3. Leung, E. et al. (1996) Immunol. Cell Biol. 74:490.
  4. Ambruzova, Z. et al. (2009) Hum. Immunol. 70:457.
  5. Tada, T. et al. (2008) Exp. Anim. 57:247.
  6. Volpes, R. et al. (1992) Hepatology 15:269.
  7. Connor, E.M. et al. (1999) J. Leukoc. Biol. 65:349.
  8. Ala, A. et al. (2001) Gut 49:3043.
  9. Yang, X.D. et al. (1997) Diabetes 46:1542.

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6056-MC
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Publications for MAdCAM-1 (6056-MC)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(1)
Bioassay
(7)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using 6056-MC Applications Species
C Lichnog, S Klabunde, E Becker, F Fuh, P Tripal, R Atreya, E Klenske, R Erickson, H Chiu, C Reed, S Chung, C Neufert, I Atreya, J McBride, MF Neurath, S Zundler Cellular Mechanisms of Etrolizumab Treatment in Inflammatory Bowel Disease Front Pharmacol, 2019;10(0):39. 2019 [PMID: 30774593] (Bioassay, Human) Bioassay Human
S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7 PLoS Pathog., 2018;14(8):e1007278. 2018 [PMID: 30153309] (Bioassay, Human) Bioassay Human
L Wang, SS DeMarco, MS Peaks, AL Maiorana-B, J Chen, MJ Crouch, BM Shewchuk, SR Shaikh, CM Phillips, LC Bridges RAR?/RXR Synergism Potentiates Retinoid Responsiveness in Cutaneous T Cell Lymphoma Cell Lines Exp. Dermatol., 2017;0(0):. 2017 [PMID: 28370539] (Bioassay, Human) Bioassay Human
Peachman K, Karasavvas N, Chenine A, McLinden R, Rerks-Ngarm S, Jaranit K, Nitayaphan S, Pitisuttithum P, Tovanabutra S, Zolla-Pazner S, Michael N, Kim J, Alving C, Rao M Identification of New Regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to alpha4beta7 Integrin Receptor. PLoS ONE, 2015;10(12):e0143895. 2015 [PMID: 26625359] (Bioassay, Human) Bioassay Human
Differential effects of ?4?7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo Gut, 2016;65(10):1642-64. 2016 [PMID: 26209553] (Bioassay, Human) Bioassay Human
Perez L, Chen H, Liao H, Montefiori D Envelope glycoprotein binding to the integrin alpha4beta7 is not a general property of most HIV-1 strains. J Virol, 2014;88(18):10767-77. 2014 [PMID: 25008916] (Bioassay, Human) Bioassay Human
Li H, Pauza CD The alpha4beta7 integrin binds HIV envelope but does not mediate bystander killing of gamma/delta T cells. Blood, 2012;120(3):698-9. 2012 [PMID: 22822002] (Bioassay, Human) Bioassay Human
Li H, Pauza CD HIV envelope-mediated, CCR5/alpha4beta7-dependent killing of CD4-negative gammadelta T cells which are lost during progression to AIDS. Blood, 2011;118(22):5824-31. 2011 [PMID: 21926353] (Binding Assay, Human) Binding Assay Human

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Bioinformatics

Gene Symbol MADCAM1
Uniprot