CCL25/TECK Antibody [Unconjugated]

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Recombinant Mouse CCL25/TECK (Catalog # 481‑TK) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

CCL25/TECK Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL25/TECK
Gln24-Asn144
Accession # O35903
Specificity
Detects mouse CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TECK is observed. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL25
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization
Publications
Read Publications using
AF-481-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL25/TECK Antibody [Unconjugated]

  • C-C motif chemokine 25
  • CCL25
  • chemokine (C-C motif) ligand 25
  • Chemokine TECK
  • Ck beta-15
  • Ckb15
  • SCYA25TECKvar
  • small inducible cytokine subfamily A (Cys-Cys), member 25
  • Small-inducible cytokine A25
  • TECK
  • TECKMGC150327
  • thymus expressed chemokine
  • Thymus-expressed chemokine

Background

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Human CCL25 cDNA has also been cloned and shown to encode a 151 amino acid protein that exhibits 49% amino acid sequence identity to mouse CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant mouse CCL25 has been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes.

  1. Vicari, A.P. et al. (1997) Immunity 7:291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL25/TECK Antibody (AF-481-NA)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow, IHC OCT-embedded, In vivo, Neut.


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Flow
(1)
IHC OCT-embedded
(1)
In vivo
(1)
Neut
(2)
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(5)
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Bioinformatics

Gene Symbol CCL25
Entrez
Uniprot