Recombinant Mouse CCL20/MIP-3 alpha Protein

SDS-Page: Mouse CCL20/MIP-3 alpha Protein [NBP2-35091]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse CCL20/MIP-3 alpha Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to CCL20/MIP-3 alpha Source: E. coli

  Uniprot ID: O89093

  Amino Acid Sequence: ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM

• Details of Functionality: CCL20/MIP-3 alpha protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR6 transfected murine BaF3 cells is in a concentration range of 0.1-10 ng/ml
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL20
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CCL20/MIP-3 alpha as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CCL20/MIP-3 alpha Protein

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3 alpha/CCL20
• MIP-3
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 1 in mouse. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL20
• Uniprot:
  • O89093()
  • O89093()
  • O89093()